Study On Genetic Diversity Of Caragana Microphylla Lam. In Inner Mongolia East Region

Caragana microphylla Lam.is an important species in the semi-arid,arid and desert regions.It has a potential utilization in North China where are rich in wildlife resources of this plant.Based on a thorough investigation on the ecological distribution of C.microphylla Lam.,ten populations of C.microphylla Lam.were selected from ten regions in east Inner Mongolia.from each region 30 samples were collected as the experimental samples.Evaluated and compared of Genetic diversities at two levels of morphology and it's DNA.Main results obtained are as follows:1.Morphological diversities among/within populations were discussed by analyzing characters such as leaves,pods,and seeds.The correlations between the phenotypic traits and ecological and geographic factors are significant.Analysis of variance for all characteristics was significantly different among populations and among individuals within population.The mean phenotypic differentiation coefficient (V_ST=0.206) shows that the variation among populations(79.4%) is significantly higher than that within populations.The result from the main components shows that the first three principal components contribute to the cumulative value reached 83.55%,respectively,on behalf of the reproductive and nutritional characteristics of phenotypic characteristics;The correlation analysis of ecological diversity indices and geographically ecological genes shows that Diversity index and latitude,elevation, ATM are significant correlation;in the Regression analysis and partial correlation analysis of geographically ecological genes,differences of phenotypic traits are highly related to geographically ecological genes;.Based on the cluster analysis,the ten C.microphylla Lam.populations investigated can be divided into three categories by phenotypic traits,the correlation between phenotypie Continental distance and geographical distance is not very close according to Mantel's test.2.C.microphylla Lam.'s DNA of high quality is distilled by SDS method,which accords with ISSR analysis.The ISSR reaction system for C.microphylla Lam.is established:PCR is 25ul,with 100ngDNA,0.6umol/L primer,0.4 mmol/L dNTPs,2.5mmol/L Mg²⁺,1.5U Taq enzyme,10×PCRbuffer(10mmol/L Tris-HCl pH8.3,50mmol/L KCl,0.001%gelatin),and response procedures: pre-5 min denaturation at 94℃,and then 45 cycles 94℃45s,and 53℃lmin,72℃1.5min,the final extension of 10 min at 72℃,after 4℃.3.The results of ISSR analysis shows that the genetic diversity among 10 C.microphylla Lam.177 (66.27%) bands are found to be polymorphic.,240 wild materials are amplified by 18 ISSR primers, The gene among populations Nm=1.8912.Higher genetic diversity is both observed at species level and population level.Its correlation with genetic diversity parameters and geographically ecological genes is not so close.Based on genetic differentiation(Gst) 20.91 percent.Base on the cluster analysis that ten populations can be divided into three categories,...