| People pay more attention to biological control technology which using beneficial microbial agents to control plants disease.Most of them are AMF,Trichoderma and EM et al. In this study,three micropropagated strawberry plants,Sachinoka,Benihoppe and Toyonoka, were used to compare the effect of AMF,Trichoderma and EM on the growth of strawberry, physiological indicators and powdery mildew resistance were considered.We hope to select beneficial microbial agents which can promote growth and improve resistance of micropropagated strawberry plants.And,we hope to provide theoretical basis of application of beneficial microbial agents on strawberry.The main results are as follows:1.Micropropagated strawberry plantlets were inoculated with beneficial microbial agents during transplanting.It was found that both the heights and leaf areas of the plants increase and the latter increases obviously.When transplanting,Carbendazim inhibits the inocula infection of AMF on strawberry plants at some degree.2.When micropropagated strawberry plantlets were inoculated with beneficial microbial agents in planting,the petiole lengths,heights and leaf areas of the plants increase at different degree.Such as AMF,Trichoderma and EM.With the developing of strawberry plants,the difference is becoming more obvious.In these three different types of microbial agents,the effect of AMF is most obvious.The growth of plants which planted in mixed substrate are much better than which transplanted in continuously cropped soil.3.The ability of runner developing of micropropagated strawberry plantlets of were expedited Benihoppe and Toyonoka which inoculated with microbial agents,AMF is more effective than EM,Trichoderma and CK.4.AMF or Trichoderma could increase the numbers of inflorescences per plant and flowers per plant,also they could improve the yield of these three strawberry plants, Sachinoka and Benihoppe and Toyonoka,per plant significantly.In addition,inoculation could improve the contents of soluble solids,lower the contents of organic acids,which all can to improve the quality of fruits.5.AMF or Trichoderma could significantly improve the chlorophyll contents in leaves of the three varieties of strawberry.The chlorophyll contents in leaves of the treated with AMF strawberry plants were generally higher than that of the plants which is treated with EM and Trichoderma.Toyonoka treated with Gi,for instance,the chlorophyll contents of is 1.45 times than CK,which is higher than plants treated with EM and Trichoderma whose chlorophyll contents about 37.7%and 24.8%.6.There is no significant effect on PAL activity of the inoculatied strawberry plants.But, theβ-1,3-glucanase activity increases,treated with Gi.Theβ-1,3-glucanase activity of Sachinoka and Benihoppe and Toyonoka plants is 2.5-5.5 times to CK(in continuously cropped soil) and 1.7-4.5 times(in mixed substrate).After plants inoculated with Trichoderma,the difference was significant among three varieties of strawberry plants at the activity ofβ-1,3-glucanase.Among them,β-1,3-glucanase activity of Toyonoka strawberry plants is the highest,which were 5.2 times(in continuously cropped soil) and 2.6 times(in mixed substrate) than CK plants.We evaluated the natural incidence of powdery mildew for Toyonoka,although the beneficial microbial agents didn't reduce the rate of natural infection of the powdery mildew significantly,The plants were significantly reduced disease index after treated with Trichoderma and AMF.Trichoderma is the most significant one.In continuously cropped soil and mixed substrate,the disease index of plants treated with Trichoderma were 36.7 and 19.2 respectively,which were 51.8%and 59.1%of the CK plants.
|
PDF Full Text Request