Study Of Different Sources Astragalus With AFLP And Metabolite Profiling

Astragalus are commonly used Chinese traditional medicine, through a long period of artificial cultivation, hybrid breeding and natural selection, have a large number of species. It is difficult to discriminate species for different sources. In this study, we combine DNA molecular markers and the metabolic markers technologies to distinguish Astragalus from different sources to solve identification difficulty, because there are a lot of differences between Astragalus-based multi-species, wild and cultivated, different regions. In this paper, we use the integration of modern molecular marker technology and the latest research metabonomics technology for the identification of Chinese herbal medicine to provide new idea and method.1. The experiment with AFLP technology analyzes 19 different sources of Astragalus mernbranaceus (Fisch.) Bge. and A. membranaceus (Fisch.) Bge. Var. mongholicus (Bge.)Hsiao. We select 11 ones from 28 pairs of selective primers through the screening, getting 85 difference stripes. MEGA software is used to cluster analysis, which the result is that A. mongholicus is in one group, A. membranaceus are divided into two groups. In general, we could distinguish two species of Astragalus. Different origin A. membranaceus concentrated by geographical distribution, and Hebei and Shandong A. membranaceus are together, and the two A. membranaceus of Jilin are together. A. mongholicus is relatively dispersed. The clustering result of wild stragalus is better, but some of the A. mongholicus disperse into A.. membranaceus.2. The experiment use modern analytical instruments (GC-TOF/MS) to analyze metabolite profiling of lipid and water phase of different sources Astragalus. ChromaTOF and XCMS (R platform) software are used for data processing. After scale and centralization by SIMCA-P software, principal component analysis (PCA), partial least squares - discriminant analysis (PLS-DA) and orthogonal partial least squares - discriminant analysis (OPLS) are done. It can be distinguished the two Astragalus species by PCA and PLS analysis. We analyze the lipid-phase of the two Astragalus species using PLS-DA, acquiring 10 biomarkers. We analyze the water phase of the two Astragalus species using PLS, acquiring 11 biomarkers. Different origin of A. membranaceus are concentrated by geographical distribution, however, A. mongholicus is relatively dispersed. Lipid phase of A. membranaceus of Jilin Tonghua and Jilin Siping is very closed; lipid phase of A. membranaceus cultivation of Hebei Baoding and Shandong Heze is very closed; water phase of Gansu Zhangxian wild A. membranaceus and Heilongjiang Hegang wild A. membranaceus is different from other A. membranaceus.It can distinguish 19 different places of the A. mongholicus and A. membranaceus using Molecular marker technology (AFLP) and GC-TOF/MS Metabolomics technology, and some characteristics of the geographic distribution are demonstrated,. A. membranaceus of various geography are relatively concentrated, and it is significant different between geographical species. The difference of A. mongholicus is relative few, and there is a cross between various geographical species. Metabolomics GC-TOF/MS technology can distinguish between wild Astragalus and cultivated. Comprehensive comparison, the results of the two analyses are accordant.