Effects Of High Tempertaure On Acetylcholinesterase And Detexification Enzymes In Insecticide-susceptible And Resistant Strains Of Plutella Xylostella

In this study, the insecticide- susceptible (S) and resistant (R) strains of diamondback moth which reared at high temperatures (36℃,40℃) lasting for different time (1,4,8,24 huors), were compared with the inhibition of acetylc- holinesterase(AChE), carboxylesterase(CarE), glutathione S-transferase(GST) and with the content of P450. The insecticide- susceptible (S) and resistant (R) strains of diamondback moth were reared at high temperature (33.5℃), then their F1 and F2 DBM were compared with the inhibition of AChE , CarE, GST, and the content of P450. The results of this thesis showed that:(1) Comparison of inhibitory of enzyme activity of the resistant and sensitive diamondback moth dealed with high temperature(36℃,40℃) at different time:The high-temperature-treament lasting for short period (1, 4 hours) has little effect either on resistant or sensitive diamondback moth. The resistant diamondback moth reared at room temperature has the similar activity of AChE, CarE with sensitive diamondback moth, while the activity of detoxification enzymes (GST and P450) was quite different between them.High-temperature at 8, 24 hours could significantly reduce the activity of these enzymes in diamondback moth, and the reducing in resistance were much markedly than in the sensitive ones.(2) Comparison of inhibitory of enzyme activity of the F1′, F2′DBM, which were reared at high temperature(33.5℃) from F0 resistant and sensitive diamondback moth.Compared of the inhibition of AchE, CarE, GST and P450, the resistant's F1 were much remarkable than the sensitive DBM's, which were both reared at 33.5℃. Compared of the third larva of F2′DBM reared at 25℃and control F0 DBM, the AChE recovered fast, and the detoxification enzymes recovered slowly.