Description Of 15 Nematode Species Of Citrus Root In Yongchun And Studies On New Technologies For Nematodes Separation

Parasitic nematodes of Citrus Roots in the production areas of Quanzhou were investigated during 2007～2009.15 species of plant parasitic nematodes belonging to 7 genus,7 families,and 3 orders were collected,observed and identified.Of them 1 new record species in China(marked with "") and 7 new record species on Citrus in China were found(marked with "☆").These species are:Pratylenchus coffeae Filipjev & Stekhoven,1941Helicotylenchus dihystera(Cobb,1893)Sher,1961☆Helicotylenchus digonicus Perry in Perry,Darling et Thorne,1959☆Helicotylenchus californicus Sher,1966☆Helicotylenchus pseudorobustus(Steiner,1914) Golden,1956Helicotylenchus exallus Sher,1966Helicotylenchus belli Sher,1966☆Criconemella curvata(Raski,1952) Lucet Raski,1981Aphelenchus avenae Bastian,1865Aphelenchus isomerus Anderson et Hooper,1980☆Aphelenchoides goodeyi Siddiqi et Franklin,1967☆Aphelenchoides rutgersi Hooper& Myers,1971☆Aphelenchoides tuzeti B'Chir,1971☆Paraphelenchus myceliophthorus Goodey,1958Xiphinema flagellicaudatum Luc,1961This article had a discussion of new technologies for nematodes separation. Through the comparison on the method of nematodes separation between Baermann funnel and nematode atomization separator device,we found the latter one not only can do a good job in separation of nematodes efficiently,but also can decrease the mortality of nematodes during the separation.At the same time,nematode atomization separator device can maintain survival rate of the samples.Traditional methods of separation were destructive methods(like Baermann funnel method) and the samples were destroyed badly.Customs and Quarantine Bureau do plant quarantine of many precious flowers and trees seedlings everyday.But the samples were reduced their vitality during the separation with the traditional method.Because of a high degree of hypoxia,most of them were even dead.This caused a tremendous waste,so nematode atomization separator device as a new method give a great help to entry-exit inspection and quarantine work.