Genetic Diversity And Relationship Of Acer L. Germplasm Resources Detected By SRAP Markers

Acer L. is an important ornamental. Molecular marker, which can detect the genetic diversity at DNA level, is a powerful tool to study genetic diversity. SRAP markers have been popularized for their simplicity, rapidity and stabilization. The genetic diversity and relationships of 31 Acer L. varieties, was evaluated according to SRAP markers in which genomic DNAs were amplified with 11 SRAP primers. The 31 Acer L. varieties were clustered into different groups according to the similarity coefficient (Nei-Li), which represented the genetic relationship of different varieties.The main results showed as follows:The method of CTAB was much better compared with other two methods applied in this experiment in extracting DNA of Acer L.,DNA obtained by which had a higher quality and purity .To establish the system of SRAP molecular marker in Acer L.,the concentration of dNTPs, Mg2+, and primers affecting the SRAP-PCR reactions were optimized.The optimum system was: template DNA 100 ng, 10×PCR Buffer (Mg2+ free), Mg2+ 2.5 mmol / L, dNTPs 0.2 mmol / L, primers 0.5μmol / L, Taq DNA polymerase 1.0 U. The total volume of reaction was 50μL.11 primer pairs producing qualified fragments were selected from 90 SRAP primer pairs. The genetic diversity of 31 Acer L. germplasm was studied by SRAP markers. The results showed that 186 bands were amplified totally by 11 pair SRAP primers, of which 183 bands (98.04 %) were polymorphic in the species level.Cluster analysis was performed by using of UPGMA method based on Nei-Li similarity coefficients, and 31 Acer L. were divided into 6 groups of similarity coefficient at 0.594. GroupⅠincluded Sect. Palmata, Ser. Sinensia of Sect. Microcarpa and the cultivars of Acer L. GroupⅡincluded A. rubrum and A. saccharinum L. GroupⅣincluded A. herryi Pax, A.'variegatum'and A.'Elegans'. GroupⅤincluded A. ginnala Maxim., A. sinopurpurascens Cheng and A. davidii Fr. GroupⅥincluded A. yanjuechi Fang et P. L. Chiu. Others were included groupⅢ. Some relationships between sections of Acer L. were supported.