The Preliminary Study About The Attraction Mechanism Of Black Poplar Volatile Compounds To Moths

In this research,we found several volatile matter have more attractive to moths through extracting,separating and analyzing volatile constituents from black poplar leaves and the biological mensurating to the attrctive of moths.Several compounds were preliminarily confirmed to be accountable for attracting moths.The results as follows:(1)A method for analyzing volatile constituents from black poplar leaves with gas chromatography/mass spectrometry(GC/MS)coupled with solid-phase microextraction(SPME)was developed.The experiment results showed that the volatile of black poplar leaves consist of 16 compounds.There were different volatile matter types,contents and diurnal rhythms of volatile released in different seasons.(2)Electroantennograms(EAG)activities of Clostera anastomsis(Linnaeu)to host volatile compounds were recorded.The result showed that there were no differences between EAG reactions around copulation and the same result of female and male.Some volatiles were found to be more attractive than other volatiles in the test.The EAG activities are obviously enhanced when the volatiles were mixed(3)The behavior responses of Clostera anastomsis(L.)to black poplar leaves volatiles compounds were tested in "Y" style olfactometer.Some volatiles were found to be more attractive than other volatiles in the test.The mixture was the more attractive to Clostera anastomsis(L.)in the olfactometer when these volatiles were mixed.(4)The lure of Clostera anastomsis(L.)to plant volatiles was determined in field..Some volatiles such as 2-hydroxy-Benzaldehyde,(Z)-3-Hexen-1-ol were found to be more attractive to moth than other volatiles in the field test.Excellent formula was tested to be the most attractive.The ratio of the attractants against control could reach 8.3:1.