Effects Of GLP-2 On The Proliferation And Function Of Weaned Piglet Intestinal-Epithelial Cells In Primary Culture

28-day-old weaned piglet intestinal epithelial cells in primary culture were selected to evaluate the effects of Glucagon-like peptide-2 on the proliferation and function of weaned piglet intestinal-epithelial cells.Firstly,the growth characteriztion of weaned piglet intestinal epithelial cells was investigated.The quantity and morphous of cells was observed by invert microscope.in the 48h,72h,96h,120h,144h and 168h,at the same time, the cell MTT absorption at 490nm wavelength was observed.Secondly,the alkaling phosphatase activities,creatine phoshhokinase activites,lactic acid dehydrogenase activites,lactic acid content,protein retention and total protein content were investigation when the weaned piglet intestinal epithelial cells was growing 96h in medium of containing 0,1×10^-11,1×10-¹⁰,1×10^-9,1×10^-8 and 1×10^-7mol/L Glucagon-like peptide-2 respectively.The results shows:1.the quantity of cell increased from 48h to 96h after cell inoculation and followed decreased;2.After addition of GLP-2 96h,the quantity of cell is significant or extremely significant more than the control group(P＜0.05),the MTT OD value is extremely significant more than the control group.Compare with the control group, the Lactic Acid concentration,total protein content,protein retention and Na⁺,K⁺-ATP enzyme activity significant or extremely significant increased in each GLP-2 groups(P＜0.05). the alkaling phosphatase activity,creatine phoshhokinase activity and lactic acid dehydrogenase activites in culture media extremely significant decreased(P＜0.01)The results indicated GLP-2 can encourage small intestine enterocyte of 28-day-old weaned pig cell proliferation,increase small intestine enterocyte of 28-day-old weaned pig cell protein retention,degrade the impaired degree of 28-day-old weaned pig cell,keep cell appearance and function Integrity.These effects is dose-dependent effects,and it has evident stage characteristic,the best density of GLP-2 on the proliferation and function of weaned piglet Intestinal-epithelial cells maybe between 1×10^-9 and 1×10^-7mol/L.