Studies On Tissue Culture Of Liquidambar Formosana, Liquidambar "Variant", Altingia Gralilipes And Semiliquidambar Cathayensis

In present study,a rapid propagation system of in vitro culture was established using the stems segments explant of Liquidambar formosana,Liquidambar "variant",Altingia gralilipes and Semiliquidambar cathayensis.The main results were as follows:1.Explant sterilizationIt was better to take budding young stem segments after about 15d in spring as explants for Liquidambar formosana,Liquidambar "variant" and Semiliquidambar cathayensis.The appropriate sterilization treatment was 75%alcoho 60s,0.1%HgCl₂ 12 min for Liquidambar formosana and Liquidambar "variant" and was alcoho 30s, 0.1%HgCl₂ 10 min for Semiliquidambar cathayensis.With regard to Altingia gralilipes,it'were better to take slight lignification stem segments as initial explants, and the appropriate sterilization treatment was the same as Liquidambar formosana and Liquidambar "variant"2.Axillary bud inductionWPM was a better basic medium for Liquidambar formosana,Liquidambar "variant",Altingia gralilipes and Semiliquidambar cathayensis in the initial culture. Among them,the appropriate axillary bud induction medium for Liquidambar formosana and Altingia gralilipes was WPM+NAA0.05mg/L+6-BA1.0mg/L,which their induction rate reaching 97.5%and 100%respectively;With regard to Liquidambar "variant" and Semiliquidambar cathayensis,the appropriate medium for inducing axillary bud was WPM+NAA0.1mg/L+6-BA3.0mg/L,which the induction rate of Liquidambar "variant" was 90.0%.The induction rate of Semiliquidambar cathayensis,however,was only 73.3%.3.Subculture proliferationThe appropriate proliferation medium for Liquidambar formosana,Liquidambar "variant" and Semiliquidambar cathayensis was WPM+NAA0.05mg/L+6-BA0.75～1.0mg/L.With regard to Altingia gralilipes,it's better to take twice-subculture approach.The initial subculture was mainly for proliferation and its appropriate medium was WPM+NAA0.1mg/L+6-BA1.5mg/L;The second subculture was mainly for getting sound tissue culture plants and its appropriate medium was WPM+NAA0.05mg/L+6-BA0.75mg/L. 4.Rooting inductionThe appropriate rooting medium for Liquidambar formosana was 1/2WPM + IBA0.75mg/L + SUC20g/L,which the rooting rate reaching 100%after 30d culture. Their appropriate rooting medium for Liquidambar "variant" and Altingia gralilipes, was 1/2wpm+NAA2.0mg/L+SUC20g/L+AC1.0g/L.Moreover,It also has been show that adding 0.01mg/L6-BA coordinated with 0.75mg/LNAA had an obvious role in promoting of rooting for Liquidambar "variant".