Study On The Evaluation And Rapid Propagation Of Cultivar Resources In Liquidambar Formosana

The genus maple(Liquidambar formosana Hance.)is the deciduous tree of hamamelididus(Hamamelidaceae).The autumn color is red,the ornamental value is high,and the genetic diversity is rich.But at present,it still lacks the evaluation of various quality resources.At present,the expansion of the specific resources of the maple fragrance is still mainly grafted and the human cost is relatively high.The cost of this paper,based on the resource evaluation of Liquidambar formosana was studied on tissue culture and rapid propagation of specific resources.In this experiment,the variation of 37 phenotypic traits of 58 fine Acer Maple clones in four places in Jingshan,Hubei,Hubei,Hong'an,Mount Huangshan Qimen,Henan and Zhumadian was analyzed and evaluated,and the tissue culture of Huang Huai 4,a special clone,was studied.The main results are as follows:(1)the statistical results of the comprehensive variance,coefficient of variation and phenotypic traits showed that the four traits were most likely to vary in the bark of the bark,the surface hair of the annual branch,the color of the color and the back of the leaf.Using SPSS 22 to extract the top three principal components,25 main traits were obtained.Using these 25 main characters,58 clones were clustered by Q.The results showed that,58 clones were divided into two,three,five and ten categories respectively.(2)the best disinfection scheme is to rinse 10 min with the flowing tap water,and then disinfect 30 s with 75% ethanol and disinfect 15 min with 10% sodium hypochlorite,and the survival rate is 62%.(3)subculture: induction and differentiation of the cotyledon and Hypocotyl of maple maple,the best medium for inducing cotyledon differentiation is MS+KT0.5+6-BA1.5+NAA0.1 or WPM+ KT1.0 +6-BA1.0+NAA0.1,and the differentiation rate of callus is 63.2% and 59.5%,respectively.The best medium for inducing hypocotyl differentiation was MS+KT1.0+6-BA0.5+NAA0.2 or MS+KT0.05+6-BA1.0+NAA0.3,and the differentiation rate of callus was 52.7% and 41.3%,respectively.(4)proliferation culture: using the true leaves of sprouting seedlings to proliferate and grow the cluster buds,the most suitable medium for Clustered Shoots proliferation is WPM+6-BA1.0+IBA0.2+NAA0.1.The results showed that adding 100 of PVP could reduce the browning of the medium.Adding 250 of the hydrolyzed milk proteincould reduce the browning rate and increase the multiplication and average bud length.(5)rooting culture: when the aseptic seedlings of the subculture are good and strong,they are moved to the rooting medium for rooting culture.The most suitable medium for rooting culture is WPM+IBA0.1+NAA0.3+AC0.2.