Elements And Proteomics Characteristics During Seed Developmental Processing Of Pinus Massoniana Lamb

Some progresses have been made on the somatic embryogenesis of Pinus massoniana Lamb with the immature zygotic embryos. However, there were more researches need to be done before mass propagation. In this paper, we report the elements and proteomics characteristics during the development of zygotes embryo of Pinus massoniana. The content of element composition and its variation of the seeds across the developmental stages were analyzed by the means of inductively coupled plasma emission spectroscopy (ICP-AES). Proteomic analysis was performed to explore the proteins expression characteristics, and the main proteins expressed at different stages were identified by mass spectrometry. The main results were as follows:1. There was a great difference in level of elemental content. The content of phosphorus was the highest, and the content of nickel is lower. In generally, the content of aluminum, magnesium and manganese rose with developmental processing; the content of potassium decreased in the early development stage and then rose; the content of nickel rose in the early development stage, and decreased slowly when develop to the columnar embryo stage. In the genotype of 312, the content of aluminum is particularly higher, boron showed a unique trend, and the content of calcium had a clear peak in stage 2.2. On proteomic level, forty-six differentially expressed protein spots were analyzed by mass spectrometry and then compared with Public Database. There were twenty-four proteins were identified, and in which there was Superoxide dismutase [Cu-Zn], Cytosolic ascorbate peroxidase, NBS-containing resistance-like protein and Thioredoxin H-type. Most of the proteins were associated with anti-oxidation mechanism; eukaryotic translation initiation factor, elongation factor tu; Heat shock protein 70 like protein, luminal binding protein, Chaperone DnaK and mitochondrial chaperonin-60 with the function of molecular chaperone; glutamine synthetase; and a few enzymes associated with energy metabolism. It was speculated that they involve in regulation of redox state, protein translation, energy metabolism, nitrogen metabolism process. The other proteins are unknown, and need to be further investigated.