The Study On Embryo Culture Of Paeonia Rockii

The embryos of Paeonia rockii were used to study the effect of different embryonic age (30 days and 45 days and 60 days and 75 days and 120 days after florescence) on germinating.Different basic medias,regulators,carbon source and CH were studied in the course of germination, multiplication and rooting of embryo and the main influence factors and effective concentration scale were analysed. The main results were summarized as follows:1. The germinating rate of young embryos (30 days and 45 days and 60 days and 75 days after florescence) which were cultured in medium(MS + 6-BA 1.0 mg/L + IAA 0.2 mg/L + 20 g/L sucrose) were 23.3 %,41.7 %,53.3 %,58.3 %.The yonger of embryonic age, the harder germinating, and the older of embryonic age ,the easier germinating. Young embryo (75 days after florescence) was easier to form plantlet by pre-chilling (4℃)15 days and germinating rate was 68.3%.2. Young embryo (75 days after florescence) was easier to form plantlet by pre-chilling(4℃, 15d). The better medium should been chosen MS(3/2 trace)+ 6-BA 1.0mg/L + IAA 0.2 mg/L + CH 0.3 g/L + glouse 10 g/L + sucrose 20 g/L + agar 6.0 g/L + PVP 0.1 g/L, and germinating rate was 100 %, plantlet forming rate was 98.3 %.3. The mature embryo of 120 days after florescence were carried on first culture that can be directly cultivated to plantlet in MS or 1/2MS. The mature embryos were not germinated in medium which had not trace element.The result demonstrate:the suitable medium were modified MS(3/2 trace) + 6-BA 1.0 mg/L + IAA 0.2 mg/L + CH 0.3 g/L + sucrose 30 g/L + agar 6.0 g/L + PVP 0.1 g/L, and germinating rate was 100%, plantlet forming rate was 95%.4. The subculture of Paeonia rockii seedlings. Monosaccharides as carbon source was better than the mixed sugar as carbon source. In monosaccharides as carbon source, the sucrose was superior to glucose. Iron content was one of the important causes in plant high of Paeonia rockii subculture.TDZ and AC to reduce the proliferation subculture multiples Miller.The medium was MS,6-BA 1.0 mg/L,NAA 0.01 mg/L,sucrose 30 g/L and the proliferation rate could reach 5.7 by 30 d later.5.Rootage of Paeonia rockii. NAA can not take root in vitro, low concentrations of IBA and IAA adding medium alone, can not take root in vitro, and mixed-use control in favor of rooting;Low concentrations of IAA and TDZ combination had a high rate of root than the combination of IBA and TDZ.The medium was 1/2MS supplemented with 0.05 mg/L TDZ, IAA 0.1 mg/L, AC 2 g/L which rooting rate could reach 26.68 % by 30 d later.