| Double dilution method was used to detect antibacterial activity of florfenicol and its combinations with other 13 kinds of antibiotics in vitro against pig escherichia coli and streptococcus. The selected antibiotic was combined with florfenicol can significantly reduced MIC and their FIC was detected.The optimal proportion of experimental copound and drug interactions was observed. The best combination in bacteriostatic effect was used as a compound drug prescription of orally emulsions; Single factor experiment method and the orthogonal experiment method was used to definite the formulation prescription of the compound florfenicol emulsion and then florfenicol oil-in-water oral emulsion.was prepared and quality evaluation was detected in particle size,thermal stability,normal temperature stability,stability of low temperature, etc. At last the term of validity for compound florfenicol emulsion.was determined。Results showed that the pig E. coli and streptococcus showed resistance to florfenicol. Most E. coli isolates show resistance to Y10. Compound florfenicol emulsion showed obvious bacteriostatic effect to the pig E. coli and streptococcus. FIC index was measured for 0.03126 ~ 0.14063 and all present for synergistic effect. Compound florfenicol emulsion that made of twain - 80 and span 80 as the main emulsifier, starch, CMC etc has very good stability. When compound florfenicol emulsion was centrifugaled 4000r/min for 15 minutes and sealed under 30±2 degrees Celsius, the relative humidity 60% of±5% conditions for 6 months ,no stratification was found. Water-solubility is good, when diluted to 2,6,and 8,10 times solution, no precipitation exist .The term of validity for compound florfenicol emulsion is about 3 years.Above results showed that the compound florfenicol emulsion show inhibition action to drug-resistant bacteria and is stable,water-soluble, convenient to use.It overcome problems in resistance, poor solubility and low bioavailability of existing florfenicol preparation.
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