Study On The Relationships Between Polymorphisms Of Chicken CACNA1S Gene And Meat Quality And Carcass Traits

142 chickens from 5 populations(Xiaoshan chicken:XS;Xianju chicken:XJ;Linkun chicken:LK;Xiaoshan chicken×Xianju chicken F1:XS×XJ;AA chicken:AA)were studied.5'-UTR～exon1 sequence of(calcium channel,voltage-dependent,L type,alpha 1S subunit)CACNA1S gene of chicken was cloned and sequenced for the first time (GenBank Accession Number:GQ184725).Single nucleotide polymorphism in the coding sequence of CACNA1S gene was detected by PCR-SSCP and DNA sequencing in 5 breeds and the effect of genotypes on meat quality and carcass traits was studied by GLM analysis.The results were as follows:(1) 5'-UTR～exon1 sequence of CACNA1S gene of chicken was cloned for the first time,and it was 1863bp in length(Genbank No:GQ184725).(2) 4 SNPs sites in 5'-UTR of CACNA1S gene were determind:213(C→T),551(G→A),716(C insert/deletion),1711(C→G).When 551(G→A),regulatory element HSF have priority,and when 1711(C→G),regulatory element ADR1 becomes to ADR1,M2F1,STRE and GATA-1.(3) It is showed that 2 allele(denoted as A,B and J,K) and 2 genotype(denoted as AA,BB and JJ,KK)was detected in SNPs213 and SNPs716 site,the frequency of 2 allele and 2 genes was 0.2254,0.7746 and 0.5141,0.4859 respectively;2 allele(denoted as M,N and X,Y) and 2 genotype(denoted as MM,MN,NN and XX,XY,YY)was detected in SNPs551 and SNPs1711 site,the frequency of 2 allele and 2 gene was 0.4683,0.5317 and 0.4648,0.5352 respectively.PIC value of 3 populations(XS,XS×XJ,AA) in SNPs213 and SNPs551 was less than 0.25,it is low polymorphism.PIC value of Others are medium polymer.(4) The results showed that the least squares means(LSM) of BMP had very significant difference between JJ and KK genotype(P＜0.01) in SNPs716,the least squares means(LSM) of EP,BMP,SEP had very significant difference between YY,XYand XX genotype(P＜0.01) in SNPs1711,the least squares means(LSM) of IMP,IMF,Glu had significant difference between MM,MNand NN genotype(P＜0.05) in SNPs551,the least squares means(LSM) of IMP,IMF,Glu had very significant difference between JJ and KK genotype(P＜0.01) in SNPs716,the least squares means(LSM) of IMF,Glu had significant difference between JJ and KK genotype(P＜0.05) in SNPs1711.The CACNA1S gene could be a candidate modifier gene that affect or control the meat quality and carcass traits of chicken.