The Study Of Actinomycete SPRI-710055 And Its Active Metabolites

Actinomycete SPRI-710055 which could produce herbicidal active metabolites was found by microbial bioscreening.This experiment was conducted to identify the strain and study its herbicidal metabolites.With the help of solvent extract,silica gel chromatography,thin layer chromatography,and high performance liquid chromatography and other ways,a purified compound with herbicidal activities was isolated from the fermentation broth,named SPRI-710055-â… ,The spectra data of UV,MS,1H-NMR,13C-NMR and others were used to identify this compound.The molecular formula of the compound was confirmed as C15H23NO4,with a molecular weight of 281.Compared with the known compounds in literatures and compounds database,the structure of SPRI-710055-â… was as same as Actidione.Its herbicidal activities were proved.The reverse phase high-performance liquid chromatography analysis method was established,which could be used to analyse Actidione in the process of the fermentation and extract.Herbicidal active of SPRI-710055-â… was tested in greenhouse pot culture .The strain was identified by 16S rDNA gene sequence analysis and the study of morphological,physiological and biochemical characteristics.The results showed that the strain SPRI-710055 belonged to Streptomcyces albulus.