Genomic Size Confirmation Of Mycoplasma Suis By PFGE

Haemotrophic Mycoplasma (hemoplasma) is a kind of uncultivated pathogen and obligates to parasite on the surface of erythrocytes of vertebrate hosts. The acute disease of hemoplasma in feeder pigs was described as febrile anemia and icterus with low morbidity and high mortality. Chronic hemoplasma infection results low reproductive efficiency in sows, growth retardation and increased incidence of complicating disease. This disease has induced big economic loss in pig farming.In the present study, Mycoplasma suis (M. suis) was experimentally infected mini-swine to establish the animal model. Blood staining and PCR were applied to confirm the experimental infection. Heat dissociation, density gradient centrifugation and filtration were performed to purify M. suis from blood cells. Further separation of M. suis genome from remaining host genome contamination was accomplished by pulse field gel electrophoresis (PFGE). The 1.9 mega base large DNA band, purified from PFGE gel, was confirmed as M. suis genome origin by DNA hybridization with 16S rRNA gene probe of M. suis. The present study confirmed the genome size of M. suis, which was expected to do contribution for sequential genomic study on M. suis.