| Using self-incompatibility line to product Brassica oleracea L.hybride is one of the main technical approaches. However, self-incompatibility line itself have low affinity and poor seed pollinated after flowering, so the self-incompatibility line have to propagate by artificial pollination in the bud stage, which needs a lot of labors, time and increases seed costs. In order to improve the index of self-incompatibility and reduce the breeding costs, in this study, five kinds of chemicals, including quercetin, DMSO, luteolin, carbonic anhydrase and molybdenum sodium were used to treat three Brassica self-incompatibility lines two weeks before flowering, the pod rate and the affinity index were investigated by artificial self pollinated after flowering, and the changes of solube proteins, ATP and POD isozymes in the stigma were analyzed by polyacrylamide gel electrophoresis, expect to find out an effective way of overcoming self-incompatibility, make the self-incompatibility lines propagation process more simple, increase the reproduction rate, provide some theoretical references in the production and application in Brassica oleracea L.. The results are as follows.1. The quercetin, DMSO, luteolin and carbonic anhydrase in individual or combination way were used to treat Brassica self-incompatibility lines 08A3,08C4 and 08370 on the bud stage. Then self-pollinated was performed by artificial after flowering. The results showed that the pod rate and affinity index in most cabbage plants were significantly higher than the flowering control without any pharmaceutical treatment, it indicated above chemicals to overcome of Brassica oleracea L. were effective.2. Molybdenum sodium had obvious harm to the cabbage plant leaves and buds. Even 0.5% molybdenum sodium can produce damage to the plant, which resulted leaves and flower buds becoming yellow slowiy in two weeks and the injure degree rose with the treated concentration increase.3. The experimental results showed that the effects of quercetin+DMSO, quercetin+carbonic anhydrase and luteolin were very obvious to the pod rate of Brassica self-incompatibility lines, which showed a significant difference compared with the artifical self-pollinated on the flowering stage (19.1%,26.47%,12.64%). The pod rates of 08A3,08C4 and 08370 were treated 52.17%, 66.65%and 40.96%separately by 1500μmol/L quercetin+2% DMSO, which were higher than the 1500μmol/L quercetin treatment with the same materials (37.65%,42.96%,32.18%) and were similar effective than the artificial self-pollinated on the bud stage (51%,66%,45%). The secondary chemical was the combination of quercetin+carbonic anhydrase, the effect of 1500μmol/L quercetin +6mg/L carbonic anhydrase was obvious, and the pod rate of 08A3 was 67.05%, which was higher than the artificial self-pollinated on the bud stage (51%); The pod rate of 08C4 was 62.96%, which was similar effective than the artificial self-pollinated on the bud stage (66%) The effects of 1500μmol/L quercetin+6mg/L carbonic anhydrase of 08A3 and 08C4 were higher than the 1500μmol/L quercetin treatment with the same materials (37.65%and 42.96%). The effect of 100μmol/L luteolin was obvious, and the pod rates of 08A3,08C4,08370 were 55.56%,58.13% and 47.47% separately, which were similar effective than the artificial self-pollinated on the bud stage (51%,66%,45%).4. The results showed in all treatments, the 1500μmol/L quercetin treatment was most effective, the affinity indexes of the self-incompatible lines 08A3,08C4 and 08370 were 1.18,1.24,1.05 separately, which showed a significant difference compared with the other chemicals and artifical self-pollinated on the flowering stage (0.54,0.45,0.39), although the affinity indexes were lower than the artificial self-pollinated on the bud stage with the same material separately (3.58,3.89,3.44). The performances of 1500μmol/L+2%DMSO and 100μmol/L luteolin were better, the affinity index of 08A3,08C4 and 08370 were 0.9,0.95,0.83 and 0.93,0.88,0.8 separately, which showed a significant difference compared with the artifical self-pollinated on the flowering stage.5. The changes of protein and isozyme in the stigma of three self-incompatibility lines 08A3, 08C4 and 08370 treated by luteolin (50μmol/L, 100μmol/L), quercetin+DMSO (1500μmol/L+ 0.5%,2%), quercetin+luteolin (1500μmol/L+50μmol/L, 100μmol/L) were alysised. The results showed the protein and ATP, POD isozyme bands were significantly different compared with the artificial self-pollinated on the flowering stage without any treatment, and band map of different varieties were different. The results showed the chemicals led corresponding changes of the proteins and ATP, POD isoenzymes in the stigma which may be the biochemical base of increased affinity index in flowering stage.
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