Screening Of Excellent Hybrids Of Pleurotus Ostreatus And Domestication Of Wild Strains Of Pleurotus

The objectives of this study were to breed new excellent strains of Pleurotus ostreatus by the technique of Mon-Mon crossing and Di-Mon crossing breeding. HP, PG30, JG3 and PG981 were four test-strains. We cultivated the hybrids and parents in the fall and spring. Vegetative mycelium was isolated from tissue of fresh fruit body of wild strains. Based on ITS region sequence alignment identified two wild strains into species, this two wild strains would be the new genetic resource.Non clamped monokaryons with two different mating types were isolated from HP, PG30, JG3 and PG981 by using protoplast monokaryogenesis techniques in this Mon-Mon mating and Di-Mon mating experiment. The hybrids of successful cross were preliminarily selected on the basis of antagonism test and ISSR markers. The grey correlation degree analysis of agronomic characters and Duncan multiple Comparison test were done of selected excellent hybrids for comparing their yield. Identified two wild strains through phylogenetic tree based on ITS region sequence alignment .one of wild strain can not domestication and cultivation; it is hope that using the heterokaryon from protoplast regeneration of this strain can be domestication.The results showed that HP, PG30 and PG981 their ration of two types of mycelium protoplast monokaryons were not equality, According to the antagonism test we get thirteen hybrids from Mon-Mon crossing and thirteen hybrids from Di-Mon crossing breeding. ISSR markers were applied for study on genetic analysis of hybrids. Forty ISSR primers were screened and five of which could be differentiating hybrid strains effectively. The amplification results showed that genetic similarity coefficient ranged from 0.31 to 0.94, and to provide strong support that Di-Mon hybrids were reality hybrids. Compare grey correlation degree analysis of eight agronomic characters with Duncan multiple Comparison test for their yield. All hybrids including their parents and wild strains were cultivated twice in a year under control condition. The hybrid strains 27, 26, 19, 37, 28 and 30 performed better than that of others in both cultivation times. The two wild strains have been identified as Pleurotus floridanus and Pleurotus sajor-caju according to the results of internal transcription space (ITS) sequence, compare with the first 10 similar generous uses in GenBank and phylogenetic tree.The results showed that both of Mon-Mon crossing and Di-Mon crossing breeding equally effective to generate new excellent strains. Application of protoplast technique was in Zhangjiajie wild strain for the fruitful cultivation.