Study On The Mating-gene Type Of Pleurotus Ostreatus And The Hybridization Breeding Of Pleurotus Ferulae

The germplasm resources of Pleuyrotus from microbiology research center had been studied and evaluated in the paper,. The suitable parents were screened to hybrid. At the same time, the cross bred was identified by molecular maker (SRAP). And the generation of hybrid was analyzed on the development character and the enzyme activity.The first, confirmed the mating genotype was the base of study and application. At present, the system of fungi booked has not been established. And the problem of same species with different names was very serious. Based on the biology species. 37 Pleuyrotus strains were classified to 8 colonies. And the 8 Pleuyrotus sajor-caju strains might be a cultivars or different varieties of the same cultivars.The second, the rare edible fungi, such as Pleuyrotus ferulae, the productive cyclewere longer than others. So through hybrid, selected the suitable generation to deducethe cycle and keep the quality. The experiment result showed that only P.sp (Taiwan)(A₂B₂ genotype) could crossing with P ferulae (A₁B₂ genotype). So the twogenotypes were studied as parents to analyze the advantage of hybrid.The third, the generation was identified by SRAP(sequence-related amplification polymorphism) maker. And it was more closed to P.sp (Taiwan). The development character between the parents and the hybrid was compared on the conditions under different PH, temperatures and illumination. At the same time, the enzyme activity was also analyzed between them. And the result showed that the development character and enzyme activity were all between the parents.The last, the model of generation evaluation was discussed with the sporocarp formation and yield related to the CMC enzyme activity. The enzyme activity was positive correlation with the sporocarp formation and the yield.