| Anubias barteri is a kind of aquarium plant. It depends on roots and division of caudexes'side buds for propagation. Because of singleness of variety and poor resistance to cold, the rate of propagation is terribly low. In this experiment, appropriate medium of the callus induction, proliferation and bud differentiation for Anubias barteri were studied. In order to obtain superior mutated callus, the effects of 60Coγray and NaN3 on Anubias barteri were discussed after the determination of suitable dosage of 60Coγray and optimum conditions of NaN3. As a result, the rapid propagation system was optimized, and the main results are as follows:1. The buds was used as explants, which was sterilized with 0.1% HgCl2 for 15min after dipped in 84 disinfection solution with concentration 1:100(84 disinfection solution:H2O) for 10min before cultured with medium. And the appropriate composition for the medium is MS ++0.5mg/L 2,4-D+0.1mg/L CPPU, which is better and appropriate for induction of proliferation and differentiation for callus by adding in 0.5mg/L 6-BA and 0.1mg/L NAA.2. The callus differentiation prefers to grow in solid culture. And the callus proliferation prefers to grow in liquid culture. Three types callus are found easy to differentiate.3. The effect of 60Coγray on callus for Anubias barteri has been tested, and the median lethal dose is equal to 30Gy. The callus will be damaged or deadly destroyed if it was treated with the radiation dosage above 80Gy. The stimulating effects of lower dose(<30Gy) were found and the highest rate(88%) of callus proliferation occurred when treated with 10Gy radiation dosage which is suitable for the induction of mutant. In contrast to the control, the height and the net photosynthetic rate of the mutant is low, and the frequence of micronucleus and chromosomal distortion are rised. As a result, a superior mutant with with long and narrow leaves is obtained by 5Gy.4. The results showed that NaN3 can cause albinism at seedling stage of Anubias barteri. And the 9mmol/L NaN3 is the median lethal concentration. In contrast to the control, survival rate, seedling height, the chlorophyll content and net photosynthetic rate of the mutant are significantly decreased, and the POD activity, SOD activity, CAT activity, and the frequence of micronucleus and chromosomal distortion of the mutant increased with irradiation dose. As a result, the Anubias barteri are obviously dwarf, which is helpful to enlarge the superficial area and promote gas exchange environment between plant material and water.5. Using the root-tip of Anubias barteri as materials, the suitable root-tip squashing method for chromosome preparation of Anubias barteri were as follows: sampling materials at 16:00pm and fixating with Carnoy's ?, pretreating with saturated p-dichlorobenzene for 2h, dissociating with 1mol/L HCl at 60℃for 3-4min, and staining with carbon fuchsin. The karyotype of Anubias barteri belongs to"2B"of Stebbins: 2n=2x=30=12m+14sm+4st.
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