Study On Growth-Control Gene Of Hair Follicle In Liao Ning New Line Of Cashmere Goat

Liaoning cashmere goat in China which has 138 state-level bird species is the protect genetic resources and one of Chinese prohibited outflow of genetic variety, There are cashmere soft, warm, white, etc., its economic value has been referred to as "soft gold", has great economic value.Cashmere goat skin follicle traits related to yield and quality of the villi have a direct impact, and the relevance of the skin follicle traits shared by a series of gene regulation, therefore, to carry out the skin hair follicle growth and development of research-related genes, the study and exploration of the laws of hair growth down important economic traits goats play an important role in genetic regulation.Homeobox genes is first found in Drosophila is about 180bp in length and highly conserved in evolution of DNA sequences, which encode homeobox protein of 60 amino acid residues for DNA binding protein, combined with the DNA in order to regulate the expression of other genes, which are basically a number of transcriptional regulatory factor family transcription factor Hox gene cluster is the existence of a large class of biological growth and development from the time and space coordination with Source shaped box gene, also known as developmental genes in embryonic development, cell differentiation, cancer cells such as physiological and pathological changes play an important role. Which, hoxc-13 gene in mouse skin has been proved to be involved in hair follicle keratin gene transcription.Therefore, the combination of hair follicle growth in the molecular control mechanism, to clarify at the molecular level control of cashmere growth based on the physiology and genetics, not only in the basic theory has important significance, as well as the growth of artificial control of cashmere goat breeding and provide a reliable the basis of scientific theory to create a huge economic value.Want to pass the test at different cashmere goat hair follicle cycle, collection of skin, hair follicles in the DNA and RNA extraction, processing, using RACE and RT-PCR method, the skin from the sheep amplified Hoxc-13 gene, for this experiment than Room to be Hoxc-13 gene promoter on the gene part of the structure and function. In the course of the experiment the skin of the accident to be pathogenic acetic acid bacteria (Granulibacter bethesdensis) gene fragments in order to further understanding of the gene in the skin of cloven-hoofed animals that exist in the function and laid the foundation for disease prevention. The experimental results are as follows:1. Amplification of the new line of Liaoning cashmere goats Hoxc-13 gene, long-801bp, coding region encoding many amino acids. Its nucleotide sequence and amino acid sequences are very conservative, with the mouse, cattle and other animals involves a high degree of homology between.2. From the Liaoning cashmere goat skin to Granulibacter bethesdensis gene fragment length 1388bp, coding region encoding 387 amino acids, and bacteria in the high similarity of this gene after subcloning analysis of repeated measurements, this gene was studied and structural analysis.