The Study Of Fungicide Active Metabolites And Characteristies Of Microorganisms

The metabolites of actinomycete SPRI-610155 have some activities against some plant disease fungi. Traced with activity bioassay, a purified compound with fungicide activities was obtained by the methods of extraction,silica gel column chromatography, thin layer chromatography, high performance liquid chromatography(HPLC) and others. The compound was identified with the spectra of MS and NMR. The molecular fomula of the compound is C43H70NO12. It's structure is elucidated same to Ascomycin which has been reported in the literature before.Three kinds of Marine actinomycetes'metabolites have been found with fungicide activities in Bioactivity Screening P10-16 strain, one of the three strains, was selected to make further studying. With the help of Centrifugation, Extraction, silica gel column chromatography, thin layer chromatography and other methods, a purified compound with fungicide activities was obtained from the metabolites of P10-16. The purified compound could control Pyricularia oryzae and other plant fungi disease. The HPLC. conditions were as follow: Hypersil ODS C18 (4.6×250nm/10μm) Column, 210nm UV detection and flow phase methanol/H2O(25/75).In addition, The preliminary determination of physiological and biochemical characters experiments for SPRI-610155 and P10-16 has been done. And the taxonomic status of P10-16 has been obtained through sequence of 16S rDNA .