| Rice is not only a very important agriculture resource, but also a model plant for biological research. Many studies into rice have been done in the fields of physiology, genomics and proteomics. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is able to monitor global changes of protein expression under different circumstances of specific tissues. Thus it is a powerful technique for research of rice's proteome.2D-PAGE has been a widely utilized technique in proteome. However, for rice, few systemic 2D-PAGE databases or online-resources are available, one of which is Rice Proteome Database (RPD, http://gene64.dna.affrc.go.jp/RPD/). RPD comprises information about proteins identified on 2D-PAGE maps of protein extracts from a wide variety of tissues and subcellular compartments of rice. Notwithstanding it contains a large volume of data gained by experimental work such as calculated properties of each protein, amino acid sequence and homology proteins, the information available is limited due to the restriction of experiment. Merely about 24.4% of the total gel spots were annotated with information from NCBI, which is not sufficient for the in-depth proteome analysis.OsPAD (Oryza Sativa Proteome Annotation Database) has been developed after an automatic annotation procedure was done. It supports not only database searching but also online analysis of users' submitting data via a user-friendly interface. OsPAD is a systemic proteome knowledge database for Oryza Sativa 2D-PAGE with integrated and comprehensive rice proteome data.
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