Erogot Alkaloids In Symbiont Of Achnatherum Inebrians And Neotyphodium Gansuense

Drunken horse grass (Achnatherum inebrians), an intoxicating grass growing at native grassland mainly in Northwestern China, symbiotic with endophyte of Neotyphodium gansuense, was studied in this paper. It mainly included analysis of the spatial distribution of ergonovine and ergine in drunken horse grass colleted from five different geographical populations of Gansu, Xinjiang and Qinghai; effects of N, P and K concentration on alkaloid concents of plants under pot conditions; and alkaloid contents from plants under field conditions. The main results summarized as followings:1. Alkaliods detection of 5 populations showed that different populations have different contents of ergot alkaloids. The contents of ergine, ergonovine and total alkaloids were highest in Hami of Xinjiang. Analysis showed that the contents of ergot alkaloids were related to the annual average temperature.2. Alkaliods detection indicated that different parts of plant contained different contents of ergot alkaloids. The contents of ergine and ergonovine were higher in theheadand leaf sheath than those in leaf blade and stem.3. Different concentrations of N, P and K treatments showed that with the increase of N concentration, the contents of ergine, ergonovine and total alkaloids showed a general increase trends, alkalod content was significantly higher at high N concentration than those at intermediate and low N concentrations (P<0.05). However, the contents of ergine, ergonovine and total alkaloids were significantly higher under intermediate and low P concentrations than those at high P concentration (P<0.05). There were no significant differences among high, intermediate and low K concentrations (P>0.05).4. Under field conditions, the results show that contents of ergonovine in head were significant higher (P<0.05) than leaf blade and stem, the contents of ergine in head and leaf sheath were significant higher than in leaf blade andshoot(P<0.05). Compared with the endophyte non-infected plant, tiller number, root length, leaf relative water content andshootdry weight of endophyte infected plants were increased significantly (P<0.05).