Establishment Of Regeneration System Of White Poplar School Intermediate Hybrid

Tree species of sect leuce duby is an excellent wind prevention and green forest species for its grow fast and adaptable. But leuce cutting propagation is very difficult, using conventional breeding methods is difficult to breed in a short time, which greatly restricted its cultivation and development. Tissue culture as an important biotechnology, has shown great potential and very broad application prospects. White poplar breeding can be short cycle, high efficiency, large-scale production and fast promotion by using tissue culture. In this study, comprehensive and systematic studies of cross-breeding of Populus species were done.By investigating the induction, dieffrentiation and rooting of adventitious buds, the best culture conditions of populus adenopoda×84K,populus hopeiensis×84K and populus hopeiensis×var.truncata were screening out which provides a basis of genetic transformation for the future.The main contents are as follows:(1) By using orthogonal L9 (34) , the best medium of stem segments and leaf induction adventitious buds of populus adenopoda×84K,populus hopeiensis×84K and populus hopeiensis×var.truncata were studied.The results showed that: 84 disinfectant 3min and 0.1% of the Hgcl2 5 min is the most suitable disinfection method. while initially cultured were conducted for propagation material, the use of cytokinin and auxin could increase the induction rate of adventitious buds. The most suitable medium when used 6-BA/NAA to induce the stem segments of populus adenopoda×84K was MS + 0.3mg/L6-BA +0.01 mg / LNAA and was MS + 0.5mg/L6-BA + 0.05mg/LNAA when induced the leaves of populus adenopoda×84K.The most suitable medium when used 6-BA/NAA to induce the stem segments of populus hopeiensis×84K was 1/2MS +0.7mg/L 6-BA +0.05 mg/LNAA and wereMS+0.5mg/L6-BA+ 0.05mg/LNAA and 1/2MS+ 0.5 mg/L 6-BA+0.01mg/LNAA when induced the leaves of populus hopeiensis×84K.The most suitable medium when used 6-BA/NAA to induce the stem segments of populus hopeiensis×var.truncata was 1/2MS + 0.7mg/L6-BA+0.05mg/L NAA and was 1/2MS+ 0.7mg/L 6-BA+0.05mg/L NAA when induced the leaves of populus hopeiensis×var.truncata.While initially cultured were conducted for propagation material, the use of cytokinin and auxin could increase the induction rate of adventitious buds. The most suitable medium when used TDZ/NAA to induce the stem segments of populus adenopoda×84K was MS+0.0015mg/L TDZ+0.05mg/LNAA and were MS +0.001mg/L TDZ+0.01mg/LNAA and 1/4MS +0.001mg/LTDZ + 0.05mg/L NAA when induced the leaves of populus adenopoda×84K.The most suitable medium when used TDZ/NAA to induce the stem segments of populus hopeiensis×84K was MS +0.01mg/LTDZ +0.1mg/L NAA and was MS+ 0.01mg/LTDZ+0.1mg/LNAA when induced the leaves of populus hopeiensis×84K.The most suitable medium when used TDZ/NAA to induce the stem segments of populus hopeiensis×var.truncata was MS+ 0.01 mg/LTDZ + 0.1 mg/LNAA and was 1/2MS+ 0.01mg/L TDZ+ 0.05 mg/LNAA when induced the leaves of populus hopeiensis×var.truncata.(2) The effects of TDZ,NAA and carbon source on adventitious buds proliferation showed that: hormone-free medium also can shoot proliferate adventitious buds, but the rate of proliferation was low. Among them, the optimal TDZ and concentrations for adventitious buds proliferation of populus adenopoda×84K, populus hopeiensis×84K and populus hopeiensis×var.truncata were 0.002mg/L, 0.002mg/L and 0.0015mg/L; while the optimal NAA concentration for adventitious buds proliferation of populus adenopoda×84K, populus hopeiensis×84K and populus hopeiensis×var.truncata were 0.1mg/L, 0.1mg/L and 0.01mg / L. The dventitious buds proliferation rate of populus adenopoda×84K, populus hopeiensis×84K and populus hopeiensis×var.truncata reached highest growth rate when the concentration of sucrose in the medium was 30g/L, and the dventitious buds proliferation rate of populus adenopoda×84K, populus hopeiensis×84K and populus hopeiensis×var.truncata reached highest growth rate when the concentration of glucose in the medium were 20g / L, 10g / L and 30g / L.(3 )The effects of NAA/IBA on rooting of adventitious buds showed that: The optimal medium for rooting of adventitious buds of populus adenopoda×84K, populus hopeiensis×84K and populus hopeiensis×var.truncata were 1/2MS +0.1mg/L NAA +0.1mg/LIBA,1/4MS+0.05mg/LNAA+0.2mg/LIBAand 1/4MS +0.05mg/LNAA +0.2mg/LIBA .The study can provided references for rapid propagation of tree species of sect leuce duby in China as well as the development of organogenesis and then carry out studies of genetic transformation and breeding.