Establishment Of Regeneration System Of Anthurium Andraeanum

1. Anthurium explants choice. Take the explants choice of anthurium andraeanum. Disscuessed disinfection effect of the different explants . The results indicated that: we taked 2.5% of Sodium hypochlorite and 0.1%of Mercuric chloride, Anthurium leaves and Petales were disinfected, this effect was very good. Rate of contamination of leaves and Petales reached 0.0%;This stems of leaf, petal and stems were disinfected ,which effect was worst, and rate of contamination reached 97.5%.In the leaves and Petales disinfection, we taked 0.1% HgCl2 8 minutes for disinfection, we finded disinfectan effect of leaves and Petales are best in all explants.2. Anthurium Callus induction.Take the leaf of anthurium andraeanum as explants, which inducted Callus of anthurium andraeanum. Discussed the different hormone, the different hormone combination, sucrose density processing and so on had influence of Callus induction .The results indicated that: the most suitable callus induction culture medium was MS+6-BA 1.0+2,4-D 0.1+KT 0.05 in dark, the leaf was cultured in this medium, The highest rate of Callus induction reached to 86.67%,and Callus growed well.3. Anthurium adventitious buds induction. Discussed the different hormone, the different hormone combination, sucrose density processing and so on had influence of anthurium adventitious buds induction . The results inducted that: in improvement Medium, taked MS + 6-BA1.0 + IBA0.3 (mg/L) in Lighting, The induction of anthurium adventitious buds growed well,and the rate of adventitious buds induction reached to 87.5%,and the average number of buds rached to5.8±1.08.4. Anthurium adventitious buds culture of growth. The results inducted that: The most suitable growth culture medium of adventitious buds was MS + 6-BA1.0 +IBA0.3(mg/L).in this medium, adventitious buds growed very fast,and the average height reached to 5.59 cm after 45 days. in the growth medium of adventitious buds, we found that adventitious buds growed well in the original callus induction medium.5. Induction of Adventitious buds roots. Main discussed the different hormone, the different hormone combination, had influence of roots of anthurium adventitious .we would induct adventitious buds to carry on the root formation medium.The results inducted that: the root of adventitious buds was suitable the induction culture medium which was 1/2MS+IBA0.7+NAA0.3(mg/L). Induction of roots was growed very early and fast in the Anthurium. It was effective that promoted early roots in this medium, comparison sturdy roots; more roots hair and vigorous plants growth.