Study On Tissue Culture Of Lysimachia Clethroides

Explored Lysimachia clethroides rapid propagation of tissue culture technology, and used toprovide a theoretical basis for industrial production L. clethroides seedlings and laid the foundation formolecular breeding in this paper. By taking the leaves, petioles and stems with axillary buds asexplants, cluster buds induction, callus induction, adventitious buds technology, proliferation, rootingand transplanting acclimation series of technical studies was conducted.The main results as following:1.0.05%HgCl,3min disinfection was the most appropriate method of L. clethroides seedsdisinfection, contamination rate was6.67%,the germination rate was20.00%,disinfection effect wasbest. When leaves was explanted as appropriate,0.1%HgCl9min disinfection was the most appropria-te method of disinfection, contamination rate was1.11%, the inductivity was70.00%, the induciblecoefficient was4.11.2. The best explant for inducting multiple shoot clumps was L. clethroides’s leaves, MS+6-BA1.0mg·L-1+NAA0.05mg·L-1culture medium was the multiple shoot clumps induction’s best culturemedium, the inductivity was80.00%, the inducible coefficient was12.00.3. Leaves was the best explant for inducting callus, MS+2,4-D0.1mg·L-1was the best inductionculture medium, the callus induction rate was100.00%, and the callus present yellow-green or green,without browning, loose texture.4. MS+KT3.0mg·L-1+NAA0.1mg·L-1culture medium was the most suitable culture mediumfor inducing callus to differentiate into adventitious buds, differentiation rate was up to100.00%, thecoefficient of differentiation was8.30.5. MS+6-BA1.0mg·L-1culture medium was the optimal culture medium of L. clethroides prolifer-ating, multiplication coefficient was9.00. Sucrose was a better proliferation of carbon source, added30g·L-1sucrose help to the proliferation of L. clethroides.6. When using1/2MS as the basic medium adding different auxin and different concentrations,the optimum rooting medium was1/2MS+NAA0.5mg·L-1, rooting rate was88.33%, the average num-ber of roots was6.47. The optimum concentration of activated carbon was1.0g·L-1, its rooting rate100.00%, the average number of roots was up to8.94.7. L. clethroides transplanting domestication method was that,under the natural light irradiation opening bottle domestication5d, planting seedlings in vermiculite: Perlite: peat (1∶1∶1) matrix.