Study On Different Inducer Improve Steroid Content Of Polyporus Umbellatus

Polyporus [Polyporus umbellatus (Pers.) Fries] is a medicinal Fungi ,which belongs to holobasidi- omycetidae,Aphyllophorales, Polyporaceae, Polyporus in the taxonomy.it shall be Polyporus dry sclerotia, are commonly used in Chinese medicine.Modern medicine study indicated that Polyporus umbellatus Fries have many pharmacological activities,for example, diuresis, anti-imflammation; anti-tumor, anti-radiation and enhancing immune function and so on,which has a good prosperity in apply and exploit in fulture. Therefore, polyporus steroid is a kind of valuable medicinal resources and beneficial to make full use of polyporus resource.steroids is biosynthetic intermediate in steroid synthetic. It can promote enzyme related to steroid synthetic product in cell of Polyporus.This topic selectedβ-sitosterol, stigmasterol and cholesterol and their mixtures as inducer, add inducers in the range of (0.05‰~ 0.20‰) to liquid fermented medium, by addβ-CD and ultrasound for improving inducer solubility.23℃suspended oscillating culture.Then testing steroid accumulation of Polyporus mycelium and broth. furthemore, three kinds of mixtures of sterol and its optimal concentration, which also compared the induction of sterol best.The results as follows:(1) single inducerβ-sitosterol and cholesterol promote the growth of mycelium of Polyporus umbellatus when theβ-sitosterol concentration is 0.020%, the best growth conditions of mycelium, concentration is 0.015%, the highest content of steroids; when cholesterol concentration is 0.020% dry weight of mycelium is biggest, the net increase amount of steroid is the highest. Stigmasterol can not promote Polyporus steroid accumulation.In a word,when cholesterol concentration is 0.020%, the largest concentration of steroids mycelium (53.224mg), so it is the best effect among the three.(2) Mixed inducerTests show that the hybrid inducer promote on the growth of mycelium of Polyporus .The amount of steroids were increased to varying degrees. Status in the growth of mycelium,when concentration of inducer as 0.005%, E group (β-sitosterol + cholesterol) dry weight of mycelium is the highest. Containing cholesterol group (E group, F Group G Group) Polyporus sterol was significantly higher than the net increase in non-cholesterol group (D group); addition to the concentration of 0.020%, the net increase of steroids was less than the other three groups,E group(β-sitosterol+cholesterol) in the other two levels are higher than the other group,and a concentration of 0.005%,the highest net increase steroid(97.338),it can be said thatβ–sitosterol and cholesterol mixed is the best inducer.The resule show that the mixed inducer was better than a single inducer .The induction effect of cholesterol is stronger thanβ-sitosterol and cholesterol,β-sitosterol and cholesterol mixture induction the best, when the concentration of 0.005%, Polyporus steroidal largest net increase, reaching 97.338mg. Polyporus steroidal medicinal value of research, make full use of Polyporus resources, the synthesis of steroidal drugs have very important meaning, so to improve the content of Polyporus steroid is necessary.