Study On Extraction And Purification Of Berberine From The Leaves And Fibrous Roots Of Coptis Chinensin

Single factor experiments and orthogonal tests were used to secelect the optimum conditions of ethanol extraction and enzyme extraction from the leaves and fibrous roots of Coptis chinensis which had lived for five years.The test materials were from Zhenping agricultural science research institute and the extraction amount of alkaloid which detected by UV spectrophotometry was used as a marker.At the same time ,the process conditions of enrichment of coptis alkaloids by D101macroporous resin were studied.The purpose of the study was to find the best conditions of extraction and purification of coptis alkaloids from the leaves and fibrous roots of Coptis chinensis in laboratory and the data should be guidance for enhancing the level of comprehensive utilization of Coptis chinensis. Obtains the main conclusion to be as follows:1.The leaves and fibrous roots of Coptis chinensis which were unutilized have large proportion of biomass.And the yield rate of alkaloids from the leaves and fibrous roots of Coptis chinensis were 5.48% and 7.1% under optimum extraction conditions.More than 50% of the alkaloid was berberine,so the leaves and fibrous roots of Coptis chinensis should be exploited.2.The best extraction conditions of reflux extraction for leaves was: the solid-liquid ratio is 10,the ethanol concentration is 70%, the extraction time was 1.5 hours, and the extraction times were 3 times;and the fibrous roots'was: the solid-liquid ratio is 12,the ethanol concentration is 80%, the extraction time was 2 hours, and the extraction times were 3 times.The yield rates of coptis alkaloids were respectively 5.48% and 7.1%.3.The best extraction conditions of enzyme extraction for leaves was:enzyme amount is 2.0%,the pH of buffer is 2.6 ,enzymolysis time was 150min in water bath which temperature was 50℃;and the fibrous roots'was: enzyme amount is 1.5%,the pH of buffer is 2.6 ,enzymolysis time was 120min in 55℃water bath . The yield rates of coptis alkaloids were respectively 4.83% and 6.01%.4.Compared with leaves ,the fibrous roots of Coptis chinensis has more alkaloid no matter which extraction method was used.Ethanol reflux extraction has higher extraction efficiency but need more time and solvent.Enzymatic hydrolysis used less time but has lower extraction efficiency. Ethanol reflux extraction was better than enzymatic hydrolysis in extracting alkaloid from the leaves and fibrous roots of Coptis chinensis can not concluded by these experiments at all.5.The enrichment conditions of coptis alkaloids by D101 macroporous resin were studied,the result showed that the best accumulation conditions were:the adsorption valus is 0.055526g/g(alkaloid/macroporous resin), sample flow rate is 1BV/h,the eluent is pH>12 30% ethanol with a 2BV/h elution speed.83.72% alkaloid was recovered under these conditions.Highly purified berberine should obtained by recrystallizing after enrichment by macroporous resin.