Genetic Analysis And Molecular Of Stripe Rust Resistance Gene In Zhongliang93447

Stripe rust, caused by Puccinia striiformis f.sp.tritici, is one of the most serious diseases of wheat in the worldwide, planting resistant cultivars is the most effective and economical methods to control this disease. Using common wheat varieties gene conferring resistance to stripe rust have a very limited role for controlling stipe rust. So many researchers have focused their attention to use of foreign genes to solve this problem. Identification and excavation new stripe rust resistance genes is an important method for controlling stripe rust. Geneticists and plant breeders pay more attention to wild relatives and elite cultivars carring durable resistance in order to search new resistant germplasms. The genetic analysis and SSR mapping of resistance genes to stripe rust in Zhongliang93447 were studied in this research, The results are as follow:1. Seedlings of the parents, F1, F2 and F3 progeny derived from the cross of Zhongliang 93447 (resistant)×Mingxian 169 (susceptible) were inoculated Race Sun11-4,CYR29,CYR30,CYR31,CYR32 and CYR33 in greenhouse to study the genetics of the resistance. Zhongliang 93447 resistant to the stripe rust were excellent. Resistance to Sun11-4 in the variety was conferred by one dominant and one recessive gene. Resistance to CYR30 and CYR31 in the variety was conferred by one dominant gene, respectively. Two dominant genes determine the resistance of Zhongliang93447 to race CYR32 and CYR33.2. Molecular mapping of one dominant gene to stripe rust resistance for CYR30 in Zhongliang 93447 were conducted. The resistant and susceptible DNA bulks were prepared from the segregating F2 population of the cross. Seven SSR markers including Xwmc813, Xwmc740, Xgwml59, Xbarc4, Xwmc616, Xwmc363 and Xbarc89 on the chromosome arm 5BS were eventually found to be linked with the gene Yr93447, respectively with genetic distances of 17,12.9,8.3,4.5,4.5,9.1 and 20.8 cM.3. This gene was assumed to be derived from Elytrigia intermedium based on pedigree analysis and molecular maker results. Comparison of this stripe rust resistance gene with the other resistance genes located on 5B suggested that Yr93447 was probably a new gene.4. Yr93447 was detected in 7% of the 43 wheat cultivars from Huang-huai wheat grown area using Xbarc4 and Xwmc616.Zhongliang93447 has excellent resistantance resourse to most of the current epidemic stripe rust race, which is important for revealing new resistant genes and wheat breeding program. The discovery of molecular markers linked to stripe rust resistance gene YrZL93447 and its application in breeding wheat cultivars from Huang-huai wheat grown area provides the reliable foundation for the utilization, the rational distribution and molecular marker assisted breeding of Zhongliang93447.