Analysis Of The Genetic Diversity In The Partial Main Sugar Beets Of Three Large Produce Areas In China By SRAP Marker

This study utilize SRAP maker to analyze the genetic diversity of 250 the representa- tion core sugar beets of three large produce areas that contain North China(Inner mongo- lia), Northwest (Xinjiang,Gansu), Northeast (Heilongjiang). It investigate the differences of sugar beet breeding materials among the region of our country. There are 66 North China beets, 90 Northwest beets and 94 Northeast beets. The main results were as follows:1. 33 pairs of pirmers were screened out from 88 pairs of pirmers used 4 sugar beets.These primers amplified distinct bands,and their polymorphism is also very high.2. 33 pairs of primers were used to analyze the genetic diversity to 66 beets that were representation in the North China. A total of 604 SRAP bands (including 319 polymorphic bands) were detected and the average ratio of polymorphic SRAP bands was 53.0%. The average genetic distance is 0.3457 and the coefficient of genetic similarity is 0.7077. The highest genetic similarity occurred between monogerm lines introduced (0.8009), followed polyembryony Diploid lines (0.7068), while the lowest one was found between polyembryony tetraploid lines (0.6592). According to the biological and economic property survey,the materials can be divided into five groups. The materials tested were divided into three groups based on cluster analysis. The genetic diversity of Northern China is rich and materials are mainly high yield, Rhizomania resistant type.3. 33 pairs of primers were used to analyze the genetic diversity to 90 beets that were representation in the Northwest. A total of 592 SRAP bands (including 324 polymorphic bands) were detected and the average ratio of polymorphic SRAP bands was 54.7%. The average genetic distance is 0.3723 and the coefficient of genetic similarity is 0.6891. The highest genetic similarity occurred between monogerm lines (0.8364), followed foreign varieties introduced (0.7528), and that between polyembryony tetraploid lines (0.7059), while the lowest one was found between polyembryony Diploid lines (0.6970). According to the biological and economic property survey, the materials can be divided into five groups. The materials tested were divided into three groups based on cluster analysis. The first group divided into three categories, the third group divided into two categories. The genetic diversity of Northernwest is rich and materials are mainly high yield and Rhizomania resistant type.4. 33 pairs of primers were used to analyze the genetic diversity to 94 beets that were representation in the Northeast. A total of 694 SRAP bands (including 424 polymorphic bands) were detected and the average ratio of polymorphic SRAP bands was 61.0%.The average genetic distance is 0.3536 and the coefficient of genetic similarity is 0.7022. The highest genetic similarity occurred between monogerm lines introduced (0.7910),followed polyembryony tetraploid lines (0.7497), while the lowest one was found between polyembryony Diploid lines (0.7101). According to the biological and economic property survey, the materials can be divided into six groups. The materials tested were divided into two groups based on cluster analysis. The first group divided into four categories, the second group divided into three categories. The genetic diversity of Northerneast is rich and materials are mainly high sugar yield and anti-brown spot type.5. 33 pairs of primers were used to analyze the genetic diversity to the 250 representa- tion sugar beets of three large produce areas. A total of 719 SRAP bands (including 459 polymorphic bands) were detected and the average ratio of polymorphic SRAP bands was 63.8%.The average genetic distance is 0.4165 and the coefficient of genetic similarity is 0.6593.The highest genetic similarity occurred between foreign varieties introduced (0.7528), followed monogerm lines (0.6945) and that between polyembryony tetraploid lines (0.6816), while the lowest one was found between polyembryony Diploid lines (0.6612). According to the biological and economic property survey, the materials can be divided into nine groups. The materials tested were divided into four groups based on cluster analysis. The first group divided into ten categories, the forth group divided into two categories.The genetic diversity of the materials is rich.The hereditary basis between North China and Northwest is near.The hereditary basis between Northeast and Northwest is far. The genetic foundation between foreign products and most domestic materials is still noticeable difference. In the dendrogram, North China materials are mainly high yield and Rhizomania resistant type; Northwest materials are mainly high yield and Rhizomania resistant type; Northeast materials are mainly high sugar yield and anti-brown spot type.