The Study Of Taxonomy And Morphology On The Pathogen Of Hypodermosis In Reindeer

In order to indentify of Hypodermatinae which were found in reindeer in Genhe of Inner Mongolia, and study the characteristics of morphology and molecularbiology. In addition, the phylogenesis and the relation of evolution of the many kinds of domestic fly larvae were studied and compared by the related gene sequence .In the morphology,the study was mainly to describe and observe the morphological characteristics of the pseudocephalon,the spines (especially the spines on the tenth ventral segment)and the spiracular plates in 2 and 3stage larvae in the unkown larva by optical microscope and scanning electron microscope . Determination of species was generally accorded to the characteristic of larvae of in stage 3 and fly morphous,and especially the latter was very important, so morphology was initially identified as Hypoderma Diana.In the molecularbiology,the species specificity sequence of mitochondrial cytochrome oxidaseI(COI)gene sequence of warble fly larvae of unknown species from reindeer in Inner Mongolia and other related fly larvae isolates were studied by molecular biology techniques. The result of DNA nucleotide sequence was confirmed that the size of species-specificity of COI gene (partial cds.) from UEA7 to UEA10 of unknown species of reindeer in Inner Mongolia Region of China was approximately 689 bp. The analysis of phylogenic tree and homology showed that the unknown species and the France isolate of Hypoderma diana were extraordinarily near to each other. Thus, the larvae of unknown species from reindeer in Inner Mongolia is Hypoderma diana.The interspecific difference showed that the sequences between Hypodermatidae and Oestridae and Gastrophilidae were obviously different, ranged from 18.7% to 31.9%. Dieffrent genus of variation ranged from12.3% to 27.2%. Different species pairwise divergences of same genus ranged from 0.9% to 18.7%; Different species pairwise divergences of fly larvae parasited in different deer ranged from 1.9% to 25.5%. Dieffrent isolates of the intraspecific variation of ranged from 0.6% to 1.2%, and intraspecific variation of Hypoderma diana was 6.0%. This study indicated that the nucleotide sequence of the mitochondrial cytochrome oxidase I(COI)gene could reflect the phylogenetic relationships among genera, species and strains to some extent.Considering the analytic result in morphology and molecularbiology,the larva founded in reindeer was Hypoderma diana.