Study On The Evaluation Of The Fermented Feed Additive Of Astragalus Membranaceus And Codonopsis Pilosula

Astrugalus menbranceus and Codonopisis pilosula were the candidates to develop animal feed additive and benefit for improving of immunity and disease resistance. Polysaccharide is one of the active components in this two tonic Chinese herbal. It was reported that there was a synergistic relationship in bio-activities between Astrugalus menbranceus or Codonopisis pilosula and the normal microflora that lived in gastrointestinal tract. This phenomenon was based on the metabolism ability of the microorganism. Fermentation is one of the processing techniques in Traditional Chinese Medicine. There are many advantages such as the drug efficiency enhancement and toxin reduction with some microorganism to ferment some Traditional Chinese Medicine. In our Lab, the previous studies showed that the total concentration was obviously increased after the Astrugalus menbranceus and Codonopisis pilosula have been fermented for 72 hours. In this study, the aim is to provide some evidences to certify the fermented feed additive of TCM was safety and high efficient. The general toxicology data, the test of weight gain and feed consumption, the blood cells and biochemistry were examined. Experiment 1 Research on the safety of FGM9 strain and the fermented product 80 mice were given the FGM9 strain by the gastric perfusion (dose: 4.5×109,4.5×107 and 4.5×105 CFU/mL) and by intraperitoneal injection (dose: 5.2×109,5.2×107 and 5.2×105 CFU/mL ) and 30 mice were given the AMF and CPF(dose:50000 mg/kg), respectively. The clinic toxic signs, the organ coefficients, the blood routine parameters and pathological changes were examined respectively. Any died cases and adverse clinical signs in the treated mice were not observed during the period of experiment. The detection indexes were not significantly different between the tested groups and the control, but the body weight was significantly increased in the AMF treated mice than that of control (P < 0.05). After the 1% AMF and CPF were added in the routine diet, the Chinese yellow-feathered broilers were fed this mixed diet for 50 days. The pathology of the organ like heart, liver, kidney and spleen was normal and health. These results suggested that the strain FGM9 and the herbal fermented products were safety and nontoxicity to mice.Experiment 2 The effect of the fermented products on the body weight gain and the feed consumption in broilers 350 1-old-day broilers were randomly divided into 7 groups: the blank control (the basal diet), the Astrugalus menbranceus control(feed added 1% Astrugalus menbranceus), the AMF groupⅠandⅠ(added 1 % and 0.5 % AMF), the CPF group I and II(added 1% and 0.5 % CPF. After the tested broilers were fed in the different diets for 60 days, the body weight gain was obviously increased at 4.58 % in mice of AMF I than that of the blank control. The feed conversion ratio was decreased at 3.94 % in AMF II and CPF I than that of the control. The results suggested that the AMF and CPF were helpful for the body weight gain and the feed conversion and the fermented herbal products are the candidates for the development of the animal feed additive.Experiment 3 The effect of the fermented products on the blood cells and the biochemistry in broilers The birds groups and treated were same as experiment 2. The test time was 7 weeks. The blood cells and part of the biochemical parameters including ALB, TP, TC, HDL-C, ALT, ALP, AST, LDH, CK, CREA, UREA, UA, Ca, P in serum were examined at 10-old-day, 30-old-day, 50-old-day. The blood sample was collected from the heart. At three detected times, the level of ALB, TC, ALT, AST, Ca, and P were markedly increased in treated groups (P < 0.05) and the level of other were different in treated groups from the control. These results suggested that the fermented herbal products were helpful to the regulation of some chemistry parameters. There was a positive relationship between this biochemical effects and the improvement of the physiological capacity and the promotion of the growth.These findings suggested that feeding the srain FGM9,AMF and CPF couldn't cause animals intoxication. Adding 1% and 0.5 % AMF & CPF to broilers'basic diet were helpful for the body weight gain and the feed conversion, and indicators of broilers′serum biochemistry were improved to a certain degree.