Separation, Purification And Identification Of Antibacterial Substance From Pleurotus Tuber-regium Fermentation Liquid

Using Pleurotus tuber-regium which comes from Sanming mycological institute of fujian, we focused preliminary on the antibacterial substances of Pleurotus tuber-regium. The results are as follows:(1) Through single factor and orthogonal experiment, we optimized the fermentation medium and culture conditions with the purpose of improving the antibacterial activity of antagonistic bacteria of Pleurotus tuber-regium.The results showed that the highest antibacterial activity fermentation medium was:soybean flour 1.5%, glucose 3%, yeast extract 0.1%, potassium dihydrogen phosphate 0.1%.Above all, glucose had an important impact on mycelial growth of Pleurotus tuber-regium. And the best optimum culture conditions was:speed 160rpm, fermentation temperature 28℃, initial pH 7.0, fermentation time 6d.(2) Using Staphylococcus aureus as the indicator strain, we studied the impact of temperature, pH, UV, and protease k on the antibacterial activity of the fermentation broth of Pleurotus tuber-regium and compared the influence of different components of organic solvents on the antibacterial effect of the extract. The results show that an inhibitory component of fermentation broth has a good thermal stability and can keep the antibacterial effect at a good extent when the pH is between 1 and 9. We also can know that the effect of ethyl acetate extract is the best of all.(3) Separating the crude extracts by silica gel column of Pleurotus tuber-regium,we obtained four components of antibacterial activity:S6, S7, S8, S9. We used S8 of which had the highest activity to Separate, and the results showed that mainly substances may be the higher alcohol,hydroxybenzene or sterol.