Safety Evaluation Of Tripterygium Wilfordii Hook. Alkaloid-Based Products To Non-Target Organisms

Alkaloids are the main insecticidal substance of Tripterygium wilfordii Hook.. They have effects of stomach poisoning, antifeedant, anaesthesia, population inhibiting and development inhibition against many kind of pests. The 1.0% T. wilfordii alkaloids ME possesses excellent insecticidal activities. It could not only kill the pests directly, but also reduce pest feeding amount, inhibit pest growth and increase pest population significantly. ME is an ideal environments harmonious and botanical insecticide used for harmless agricultural products. This dissertation determinated the toxicity of 95% total alkaloid and 1.0% T. wilfordii alkaloid ME to non-target organisms according to"Experimental Guideline for Environmental Safety Evaluation of Chemical Pesticide". The main results and conclusions are as follows:(1) Both 95% total alkaloid and 1.0% T. wilfordii alkaloid ME had no apparent contact toxicity to bee. The intake toxicity LC₅₀ of 95% total alkaloid and 1.0% T. wilfordii alkaloid ME to bee were 89.0324mg/L and 301.7481 mg/L respectively. 95% total alkaloid was moderate toxic to been. 1.0% T. wilfordii alkaloid ME was low toxic to bee.(2) T. wilfordii alkaloid-based products were moderate toxic. Both of them had no obvious contact toxicity to silkworm, but there were some impact on the weight of molting silkworm and cocoon.(3) The LD50 of 95% total alkaloid was 27.8580mg/kg to quail. The LD50 of 1.0% T. wilfordii alkaloid ME was 52.7414mg/kg to quali. They belong to moderate toxic and low toxic respectively. The accumulative coefficient of two products was 5.96. No significant cumulative toxicity to quail was found.(4) The T. wilfordii alkaloid-based products were both low toxic to earthworm. Their LC₅₀ were higher than 1000mg/L.(5) 95% total alkaloid was low toxic to tadpole, while the ME was moderate toxic to tadpole with LC₅₀ (48h) of 0.6915mg/L.(6) Water culture method showed that EC10 of ME to corn, cucumber and cowpea was 66.2499, 15.4960 and 25.8104mg/L. The ME was low risk to succeeding crops.(7) The effect of T. wilfordii alkaloid-based products on total CO2 emission from soil by the method of airtight showed that products were low toxic. The botanical pesticide T. wilfordii alkaloid-based products were low toxicity to environmental organism, more security. There will be no serious impact on the environment organisms during application. But we should be caution while used in the mulberry.