Classification Of Flies Species Around Camel's Living Environment In Inner Mongolia And COI Gene Comparision

To study the possibility of some kinds of flies served as the vectors of Parabronema skrjabini, the flies species and their distribution in camels living environment were investigated in the prairie. Also, we tried to find out the relationship among the flies by molecular biological method. Firstly, the flies were collected from three areas of deserted grasslands between July and September from 2007 to 2009 in Inner Mongolian Region. Then, they were identified and classified by conventional morphological method. Meanwhile, genome DNA of the flies was exstracted and COI genes were amplified with universal primers(UEA3 and UEA10)of files. Later, cloning and sequencing of the PCR products were done. The COI sequence was analysed by software DNASTAR, then phylogenetic tree and homology were also established.The result showed that the 25 investigaed flies species belonged to 17 genera of 7 families and another 2 families were not identified in genera level. Among all of them,12 species were firstly reported in Inner Mongolia Region. The prevalent flies were Hydrophoria ambigua up to 37.2%, followed by Haematobia titillans(23.0%)in Bayannaoer district. In Alasan district, the highest percent of flies belonged to Coelopidae (about 31.5%), then Norellia striolata (26.5%), and other species were a few. But, the dominant flies species belonged to Trixoscelidae (around 43.4%) from Wulanchabu district, followed by Lispe litorea and L. consanguinea (about 10.6% and 12.4%).Seen from the result of molecular taxonomy, the variability of nineteen COI sequences was from 0.1% to 22.8% and homology was between 78.2% and 99.9%. The differences in most of flies from various families and various genera of the same family were significant. In the nineteen COI sequences, containment of A+T was from 67.44% to 72.62% and it was from 27.38% to 32.56% for C+G, while the former was significantly higher than the later. There were 481 variation locuses in the COI sequences.The composition of flies was ascertained in living environment of camels in Inner Mongolia Region, which provided important data for investigating the vectors of Parabronemosis. The phylogenetic relationship of flies were understood, which made up a deficiency in related field and established a foundation for further research.