Differential Gene Expression Of Tomato Leaves Under Simulated Acid Rain

Acid rain caused a great damage on plants. Our national economy especially the agricultural production suffered a greater impact because of the acid rain damage. At present, the reports of tomato under the stress were mainly about drought, cold and salt stress, but study on the plant abiotic stress response mechanism, the dynamic changes of gene expression profiling on tomato under the condition of acid rain have not been reported.Acid rain damage to the plant responses on leaves the first. In this study, tomato leaves which treated with simulated acid rain were used as experimental material, used cDNA-AFLP technique to separate differentially expressed gene fragments. In this study, a fingerprinting approach-amplified fragment length polymorphism (AFLP) was done using selective base pairs, including 8 Mse I primers and 8 EcoR I primers, 86 differential expression transcript-derived fragments (TDFs) were obtained, of which 45 TDFs were cloned and sequenced. The result revealed that using database homology searches, 31 TDFs were putative gene, involving cellsignalling (TDF2,TDF4,TDF18,TDF24,TDF30 ,etc.), transcription regulation-related protein(TDF7,TDF11,etc.), stress- inducible proteins(TDF5,TDF8,TDF27), etc., the remaining 14 sequences alignment showed no homology or homologous low. According to research published about stress resistance, 8 TDFs may be associated with acid rain-stress.In order to provide reliable theoretical basis for improving the acid adaptation of tomato, this paper analyzed the acid adaptation mechanism of tomato by studing the differential gene expression of tomato leaves, treated by simulated acid rain. In addition, tomato is a model plant, this study can also provide theoretical basis for the further study on the acid adaption mechanism of horticultural plants.