Isolation Of MeJA-induced Ginsenosides Biosynthesis Associated Genes By CDNA-AFLP Fingerprint Technique

Ginsenosides have various pharmacological activities with a wide spectra of usefulness, to dissect the ginsenoside biosynthetic pathways and their molecular genetic mechanisms in depth became important premise for the development of in dustrial manufacturing biotechs.Studies have shown that MeJA is a chemical elicitor,which is able to improve the ginsenoside production.In fact, this suggested that the differential temporal expression of ginsenoside biosynthase and/or transcriptional regulator genes might play important roles in ginsenoside biosynthesis.In this experiment,we collected ginseng from Changbai Mountain, Jilin province, used it as the explant material.Ginseng callus cultures have been successfully induced and stably subcultured from one generation to another , and are maintained in a modified MS medium.Ginseng suspension cell cultures have also been successfully established. In this study,cDNA-AFLP analysis was employed to identify genes that exhibited a modulated expression following 200μM MeJA treatment in Panax ginseng grown in suspension culture.A total of twenty-eight unique TDFs was found as MeJA responsive. sixteen of them showed significant homology to genes with known or putative function,six TDFs were homologous to uncharacterized genes, while six TDFs did not show significant matches. Twenty-eight genes of known or putative function were transcriptional factors, expression regulators, and stress responding and transport facilitation genes, as well as genes involved in cellular metabolism and organization and the photosynthetic process, suggesting that a multitude of processes are implicated in MeJA response.