| Cinnamomum camphora is a evergreen shrub,which belongs to Lauraceae genus, Cinnamomum family. Cinnamomum camphora is subtropical region of China's major timber species and special economic. In this experiment basic materials is eight camphor clones, plants is obtained by vegetative propagation. In the tissue culture process, we can choose the optimization of each training session and train high quality, breeding ability, and reproduction in large quantities, adaptable, higher energy utilization efficiency of the plant, not only for the factory to provide a theoretical basis for breeding, but also for seedling market high-quality nursery stock. the experimental results are as follows:1.The axenic system of Cinnamomum camphora was establishment.The showed that the disinfection method of stem tip which 75%alcohol disinfected 8 seconds first,then deeped in 0.1%HgC12 to disinfect 9 minutes is the best. the disinfection method of central stem which 75%alcohol disinfected 10 seconds first,then deeped in 0.1%HgC12 to disinfect 10 minutes is the best. the disinfection method of the base of its stems which 75%alcohol disinfected 20 seconds first,then deeped in 0.1%HgC12 to disinfect 15 minutes is the best.2. Cinnamomum camphora screen the initial medium. Experiment selected the best initial medium for the A-14. A-14 was improved 1/2MS+2.5mg.L-1BA+0.4 mg.L-1NAA+0.8 mg.L-1AC+30g.L-1 sucrose.In the initial medium A-14 the explants budding fast,green seedlings,and the high rate of proliferation.3.AC control the browning was the best of all,in the medium containing 1.0mg.L-1AC,which control effectively the browning.the rate of browning is 11.04%.4.Effect of contamination rate,launch rate on different months of Cinnamomum camphora.the lowest months of contamination rate is march. launch rate up to 90%. contamination rate is 50% or more in june and july,which is not suitable for initial testing of axillary bud.5.The subculture of Cinnamomum camphora.using orthogonal design,and selected the first subculture medium for the best was improved 1/2MS+6-BA3.0 mg.L-1+NAA0.4 mg.L-1+agar6.5g.L-1+sucrose30g.L-1 .multiplication coefficient will up to 8.1.6.Effect of times of the subculture and light intensity for the subculture of Cinnamomum camphora. multiplication coefficient of the second subculture is maximum,so seedlings can be breeded in the second subculture.btowning rate up to 10% , mortality rate up to 10% and multiplication coefficient up to 8.1 when light intensity is 4000lx,this processing method is the best.7.The rooting culture of Cinnamomum camphora.the best medium of rooting culture is selected in experiment of the rooting culture.the best medium is improved 1/2MS+0.1 mg.L-1IBA+1.0 mg.L-1IAA+0.4 g.L-1AC,the rooting rate up to 95%.8.Effect of light intensity and light wave on the rooting ratio and rooting activity of Cinnamomum camphora. The rooting rate of tissue culture seedling up to 96% when the light wave is 4000lx.red wave great effect rooting activity of Cinnamomum camphora. rooting activity in red wave is better than others light wave.9.The transplantion of Cinnamomum camphora. Effect of different media on transplanting of Cinnamomum camphora,survival ratio of transplanting up to 91.67%in the media(yellow soil:sand: vermiculite=1:1:1).The better transplantation environment is the shed with interim mist and survival rate of transplanting up to 82.22%.10.Callus induction of Cinnamomum camphora.cullus induction of stem is more easier than petiole, cullus induction of petiole is more easier than leaf.so we choose stem which serve as material of cullus induction. Optimal disinfection method of stems that disinfected 20 seconds first in 75%alcohol,then deeped in 0.1%HgC12 to disinfect 9 minutes is the best. cullus .the medium of induction higher ratio is as follow: improvedMS+2,4-D1.0mg.L-1+NAA1.0mg.L-1+TDZ0.5mg.L-1. the optimal medium of cullus subculture is as follow ,improve MS +1.5 mg.L-1 TDZ +0.5 mg .L-1 NAA +30g.L-1sucrose.11. Comparison of Chlorophyll Fluorescence parameters of eight clones . utility rate of luminous energy, Light energy conversion efficiency and quantum yield of eight clones have different order.the order : pc -2 clone, pc -1 clone , pc -706 clone,B-7-3 clone, pc -707 clone , pc -702 clone , pc -711 clone , pc -703 clone. pc -2 clone. pc -1 clone and pc -706 clone have pnmax.12. Comparison of germination ratio of initial medium of eight clones. germination rate of initial medium of eight clones from bigness to smallness was pc -703 clone, pc -2 clone, pc -1 clone, pc -711 clone, B-7-3 clone, pc -707 clone, pc -702 clone, pc -706clone.13. Comparison of multiplication coefficient of eight clones. multiplication coefficient of eight clones from bigness to smallness was pc -703 clone, pc -711 clone, pc -2 clone, pc -1 clone,B-7-3 clone, pc -707 clone, pc -702 clone, pc -706 clone.14. Comparison of rooting ratio and root vigor of eight clones. rooting ratio of eight clones from bigness to smallness was pc -703 clone, pc -711 clone, pc -2 clone, pc -1 clone, pc -702 clone,B-7-3 clone, pc -706 clone, pc -707 clone. root vigor of eight clones from bigness to smallness was B-7-3 clone, pc -1 clone, pc -703 clone, pc -707 clone, pc -711 clone, pc -2 clone, pc -706 clone, pc -702 clone.
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