Study On Specificity Of Ldh Isozyme In Plateau Pika (ochotona Curzoniae) Tissues

Plateau pika (Ochotona curzoniae) is native to the Qinghai-Tibet plateau. To study their adaptive mechanisms, the expression patterns of lactate dehydrogenase (LDH) isoenzymes spectrum in their tissues were measured through polyacrylamide gel electrophoresis (PAGE). Our results indicated that, in mating season, six bands of LDH isoenzymes were showed in tissues of plateau pikas, the expression patterns were identical to that in testes and spermatozoa. It was showed that five bands of LDH isoenzymes in tissues of Sprague-Dawley rats, but six bands in testes. The third band in LDH isoenzymes spectrums in testes of Sprague-Dawley rats was LDH-C4. We speculated that, Ldh-c gene was not only expressed in testes of plateau pika, but also in somatic cells. To prove the inference, we cloned the full-length sequence of Ldh-c, determined whether Ldh-c was expressed in somatic cells, and explored the effects of elevation on the Ldh-c expression of plateau pika. The expression patterns of lactate dehydrogenase (LDH) isoenzymes spectrum were measured through polyacrylamide gel electrophoresis (PAGE); The Ldh-c gene was sequenced and analyzed by molecular and bioinformatics approach; the mRNA expression levels in different altitudes (3200 and 4266 m) were detected by real-time PCR.The results showed that six bands of LDH isoenzymes were showed in tissues of plateau pikas .The full-length pika Ldh-c cDNA was 1624 bp with a 999 bp open-reading frame encoding a peptide of 332 amino acids, a 106 bp 5' untranslated region (UTR), and a 519 bp 3' UTR. The coding sequence of pika Ldh-c cDNA and LDH-C subunit shared 77-86% and 74-88% homology to that of rat, mouse, cow and human, respectively. The mRNA expression level in 3200 m was obviously higher than that in 4266 m, while in somatic cells Ldh-c gene was not found.In conclusion, these results suggested that the plateau pika Ldh-c gene is not expressed in somatic cells. Six bands of LDH isoenzymes in all tissues Needs for further research. Although plateau pika is a high hypoxia tolerate mammal with high ration of oxygen utilization to cope with plateau hypoxia, hypoxia has some effects on the fertility of plateau pika. We speculated that, with the elevation increasing, the plateau pika Ldh-c gene expression was inhibited, which affected their reproduction.