Study On Specificity Of VEGF Gene In Plateau Pika(Ochotona Curzoniae) Skeletal Muscle Tissue

At the Qinghai-Tibet plateau Plateau pika （Ochotona Curzoniae） is nativeanimal, vascular endothelial growth factor （VEGF） can promote capillaryproliferation, and the microvessel density （MVD） of animal tissues is related to theiradaptility to hypoxia. To study their adaptive mechanisms, the expression patterns ofVEGF in plateau pika skeletal muscle tissues were measured through real-time PCRand the MVD of skeletal muscle of plateau pika in different altitudes were measuredby immunohistochemical staining and the correlation relation of MVD with VEGFexpression was analyzed by SPSS17.0. Our results indicated that, VEGF isoforms inplateau pika skeletal muscle is mainly composed of VEGF₁₈₉, VEGF₁₆₅ and VEGF₁₂₁.There was not significantly difference between the expression of VEGF₁₈₉, VEGF₁₆₅,VEGF₁₂₁ mRNA （respectively0.405±0.068,0.361±0.089,0.158±0.133） in plateaupika at high altitude and the expression of VEGF₁₈₉, VEGF₁₆₅, VEGF₁₂₁ mRNA（respectively0.413±0.061,0.388±0.039,0.282±0.100） at low altitude. MVD（606.61±21.16） in high altitude plateau pika was significantly higher than that（476.09±32.96） of skeletal muscle in low altitude plateau pika （P<0.05）. VEGF₁₆₅was significantly correlated with MVD at high altitude.In conclusion,at high altitude the expression of VEGF₁₈₉ is significantly higherthan VEGF₁₂₁,and the expression of VEGF₁₆₅ is significantly higher than VEGF₁₂₁ inplateau pika skeletal muscle tissues.In low in altitude the expression of VEGF₁₈₉ issignificantly higher than VEGF₁₂₁. So the VEGF₁₈₉ have a certain effect to adapt tohypoxia. And the VEGF₁₆₅ is play a role to promote capillary proliferation.