Cloning And Analysis Of Rice Majority Agronomic Traits Function Genes

Rice is one of staple cereal crops in worldwide. Stable rice production influences provision of food in global. Study on functional genomics and molecular basis of major agronomic traits and building putative architecture is significant to increase rice yield.This study reported a rice polytiller and dwarf mutant, Oshyl1, which generated from T-DNA insertion. The corresponding gene was localized to a homologous gene HYL1 in a Arabidopsis thaliana. The double strand RNA binding protein HYL1 is necessary for microRNA biogenesis in plants. Translation of OsHYL1 is terminated in advance by a T-DNA insertion and influence the biogenesis of microRNA. Thereby, regulate the development of rice. This mutant shows some complicated agronomic traits in tiller,stalk and Drastically decrease maturing rate. RT-PCR and Northern blot result shows that gene expression level slightly decreased in Oshyl1 and prominent decreased in transgenosis rice which amiRNA silence OsHYL1. Indicate that OsHYL1 direct the biogenesis of rice microRNA and regulate the architecture building of rice.Also reported a rice liguleless mutant, Oslg1-3, which was generated from early indica rice variety " Guanglu'ai 4" through the irradiation using 60Coγray. The trait of ligulelessness was inherited in a single recessive gene model and the corresponding gene OsLG1 was localized to chromosome 4 and isolated via mapping-based cloning method. localizing on Os04g56170 which reported as OsLG1. We named this mutant as Oslg1-3 for make a distinction between Oslg1-2 and Oslg1-3. Besides the absence of ligule, the leaves of the mutant were erect because of low degree of bending between leaf blade and sheath, so the mutant can be utilized to breed new rice varieties with erect leaf for reasonable dense planting. The analysis of field production indicated that the genetically improved Oslg1-3 lines can significantly increase grain yield through dense planting, it's increase the yield more 20% than wt while cultivate 50 individual plant per square meter. that demonstrated the mutant was valuable in rice breeding.