Differential Expression Analysis Of ME1, MDH1 And MDH2 Genes In Porcine Adipose And Muscle Tissues

To investigate the mRNA abundance of porcine malic enzyme 1 (ME1), malate dehydrogenasel and 2 (MDH1 and MDH2) genes across the adipose and muscle tissues at different body site of Landrace (a leaner Western breed) and Rongchang pigs (a fatty indigenous Chinese breed), The real-time fluorescence quantitative PCR was applied. In addition, the relationships betwin on adipose deposition and muscle growth traits and gene expression changes are analysed.Our results showed that the Rongchang pigs had higher adipocyte deposition capability than that of the Landrace pigs in backfat thickness (BFT), porcine obesity index (POI) and fatty acid composition. The expression changes of porcine ME1, MDH1 and MDH2 mRNA in the adipose and muscle tissues exhibited the specific different patterns by genderes and breeds:(1) The mRNA abundance of ME1 mRNA in male Landrace pigs was significantly higher than in female Landrace pigs in upper layer of back fat, lesser omentum and intermuscular fat (P<0.01), but the mRNA abundunce of ME1 mRNA in female Landrace pigs was significantly higher than in male Landrace pigs in lesser omentum and leaf fat (P<0.01). The mRNA abundunce of ME1 mRNA in female Rongchang pigs was significantly higher than in male Rongchang pigs in upper layer of back fat and pericardial fat (P<0.05), the mRNA abundunce of ME1 mRNA in male Rongchang pigs was significantly higher than in female Rongchang pigs in lesser omentum (P< 0.01).In upper layer of back fat, the mRNA abufidunce of MDH1 mRNA in male Landrace pigs was significantly higher than in female Landrace pigs (P<0.05), however the mRNA abundunce of MDHl mRNA in female Landrace pigs was significantly higher than in male Landrace pigs in intermuscular fat (P<0.01); the mRNA abundunce of MDH1 mRNA in female Rongchang pigs was significantly higher than in male Rongchang pigs in intermuscular fat, upper layer of back fat, leaf fat and pericardial fat (P< 0.01).In addition psoas major muscle, the mRNA abundunce of MDH2 mRNA in male Landrace pigs was significantly higher than in female Landrace pigs (P< 0.01), the mRNA abundunce of MDH2 mRNA in female Landrace pigs was significantly higher than in male Landrace pigs in intermuscular fat, lesser omentum, leaf fat, pericardial fat and longissimus dorsi muscle (P<0.01).(2) The mRNA abundunce of ME1 niRNA in male Landrace pigs was significantly higher than in male Rongchang pigs in lesser omentum and intermuscular fat (P<0.01), however, the mRNA abundunce of ME1 mRNA in female Rongchang pigs was significantly higher than in female Landrace pigs in inner layer of back fat and pericardial fat (P<0.05). The mRNA abundunce of MDH1 mRNA in female and male Rongchang pigs was respective significantly higher than in female and male Landrace pigs in abdominal fat, intermuscular fat and pericardial fat (P<0.01). The mRNA abundunce of MDH1 mRNA in female Rongchang pigs was significantly higher than in female Landrace pigs in upper layer of back fat, lesser omentum and pericardial fat (P< 0.01). The mRNA abundunce of MDH2 mRNA in male Rongchang pigs was significantly higher than in male Landrace pigs in longissimus dorsi muscle (P<0.01), the mRNA abundunce of MDH2 mRNA in female Rongchang pigs was significantly higher than in female Landrace pigs in lesser omentum, pericardial fat, intermuscular fat, leaf fat and psoas major muscle (P<0.01).