Expression Changes Of Caveolin Family Genes In Adipose Tissue And Muscle Tissue Of Porcine

To investigate the developmental expression patterns of Caveolin Family Genes at the age of 7 months in fat tissue and muscle tissue of Landrace(a leaner Western breed) and Rongchang pigs (a fatty indigenous Chinese breed), Real-time fluorescence quantitative PCR was applied. In addition, the genes expression difference between breeds and ages and the effects on adipose traits were analyzed.Our results showed that the Rongchang pigs had higher adipocyte deposition capability than that of the Landrace pigs in backfat thickness (BFT), porcine obesity index (POI) and fatty acid composition. The expression changes of porcine CAV1, CAV2 and CAV3 mRNA in the adipose and muscle tissues exhibited the specific different patterns by genderes and breeds:(1)The mRNA abundance of CAV1 mRNA in male Landrace pigs was significantly higher than in female Landrace pigs in leaf fat. (P<0.01). The mRNA abundance of CAV1 mRNA in male Landrace pigs was significantly higher than in female Landrace pigs in intermuscular fat. (P<0.01). The mRNA abundance of CAV1 mRNA in male RongChang pigs was significantly higher than in female RongChang pigs in intermuscular fat. (P<0.01); The mRNA abundance of CAV2 mRNA in male Landrace pigs was significantly higher than in female Landrace pigs in leaf fat. (P<0.01). The mRNA abundance of CAV2 mRNA in female Landrace pigs was significantly higher than in male Landrace pigs in intermuscular fat. (P<0.05); The mRNA abundance of CAV2 mRNA in male RongChang pigs was significantly higher than in female RongChang pigs in Inner layer of back fat, Abdominal fat and Intermuscular fat (P< 0.05). The mRNA abundance of CAV1 mRNA in female RongChang pigs was significantly higher than in male RongChang pigs in Inner layer of back fat. (P<0.01). The mRNA abundance of CAV3 mRNA in female Landrace pigs was significantly higher than in male Landrace pigs in leaf fat (P<0.01)(2)The mRNA abundance of CAV1 mRNA in female RongChang pigs was significantly higher than in female Landrace pigs in leaf fat and Inner layer of back fat. (P<0.01); The mRNA abundance of CAV1 mRNA in male Landrace pigs was significantly higher than in male RongChang pigs in leaf fat and Intermuscular fat (P< 0.01); The mRNA abundance of CAV1 mRNA in male RongChang pigs was significantly higher than in male Landrace pigs in Lesser omentum (P<0.01).The mRNA abundance of CAV2 mRNA in female RongChang.pigs was significantly higher than in female Landrace pigs in leaf fat and Inner layer of back fat (P<0.01); The mRNA abundance of CAV2 mRNA in male RongChang pigs was significantly higher than in male Landrace pigs in Abdominal fat and Inner layer of back fat (P<0.05); The mRNA abundance of CAV2 mRNA in male RongChang pigs was significantly higher than in male Landrace pigs in Upper layer of back fat (P<0.01). The mRNA abundance of CAV3 mRNA in female Landrace pigs was significantly higher than in male RongChang pigs in leaf fat (P<0.01).The results that the CAV1 and CAV2 might be two candidate gene for carcass.CAV3 to some extent affect adipose deposition and might be a candidate gene of meat production trait.