Study On Application Of PGPR Fertilizer On Floating System Of Flue-cured Tobacco And Production Of Flue-cured Tobacco

High-effective antibiotic bacterium B03, bacterium of nitrogen fixation N05, bacterium of dissolving phosphorite P04 and bacterium of dissolving potassium feldsper K03 were separated from the tobacco rhizosphere and mixed to PGPR fertilizer after strain identification to reduce the use of chemical fertilizer and poison, promote the output and quality of the tobacco.1) Strain identificationIt can be concluded that antibiotic bacterium B03, bacterium of nitrogen fixation N05, bacterium of dissolving phosphorite P04 and bacterium of dissolving potassium feldsper K03 belong to Bacillus and maybe Bacillus cereus, Alcaligenes and Alcaligenes faecalis, Enterobacter and Enterobacter cloacae, Enterobacter and Enterobacter asburiae, respectively with phylogenetic tree based on 16S rDNA sequences and the physiological and biochemical reaction. Four bacteria are all not pathogens, can be used for the production of PGPR fertilizer.2) The production of PGPR mixed fertilizerDuring the antagonistic experiment, no antagonism had been found except the antibiotic bacterium B03 exerts weak antagonistic reaction on bacterium of dissolving phosphorite P04. PGPR mixed fertilizer was made and the live bacteriums was no less than 100,000,000 which fits to the national standard.3) The application of PGPR mixed fertilizer in tobacco floating systemMushroom substrate were used to floating system and revealed that Agaricus Bisporus mushroom substrate could take the place of peat in tobacco seedling production after optimize the treatment before seedling and seedling condition, but required further confirm that whether the Pleurotus ostreatus mushroom substrate also can be used. The PGPR fertilizer improved rate of emergence by 3%, the agronomic characters of tobacco seedling as well as strong seedling. The fertilizer had been promoted the root growth greatly, for example, Agaricus Bisporus mushroom substrate with PGPR fertilizer raise to 101.7%.4) The application of PGPR mixed fertilizer in flue-cured tobacco fieldThe influence on the tobacco-planting soil and the production of flue-cured tobacco were studied employing PGPR fertilizer and reducing the use of the chemical fertilizer when flue-cured tobacco transplanted in plots. The results showed that application of PGPR fertilizer would increase the number of rhizo-bacterium of dissolving phosphorite significantly in budding period (p<0.05) and reduce amount of actinomycete during growth period and have no influence on total amount of bacteria, fungus, nitrogen-fixing bacteria and dissolving potassium bacteria. PGPR fertilizer also increased the tobacco rhizo-microbial biomass C contents, for example, 22.2% had been increased in budding period, a period of strong physiological activity. The use of PGPR fertilizer improved effectiveness of mineral element, such as N, P, K, Ca, Mg, Cu, Zn, Mn, Fe were all significantly higher than without GPGR fertilizer, range from 8.19% to 49.33%, while the effectiveness of B reduced. After PGPR fertilizer application, the disease incidence in flue-cured tobacco decreased and tobacco yield per 0.067 ha and production value increased up to 7.59% and 14.8%, respectively. It is well known that reducing N, P and K fertilizer would result for low yield, quality and production value of tobacco, but the production value of application of PGPR fertilizer while reducing N, P, K fertilizer increased up to 16.44%, it can be concluded that the use of PGPR fertilizer could make up for the decreased chemical fertilizer.