Dynamic Study On Effects Of Soybean Oligosaccharide On Microbial Flora In Intestinal Tract Of Duck

This study was designed to research the effects of SBOS in different levels on growth performance and intestinal microbial community of Cherry Volley Duck. An experiment was conducted with 300 Cherry Volley Ducks aged 1-day that were randomly divided into five groups and 5 replicates each treatment, which were fed with basal diet (control group), basal diet+antibiotic (group 2), basal diet+SBOS in 300mg/kg level (group 3), basal diet+SBOS in 500mg/kg level (group 4) and basal diet+SBOS in 700mg/kg level (group 5). The experiment consisted of two parts, the first period (1-14days) and the second period (15-28day).BW, ADG, ADFI and F/G were examined in each weekend.3 Cherry Volley ducks randomly selected from each group were slaughtered to obtain the caecum content in each weekend and T-RFLP was used to detected the microbial flora. The results showed that:1. Effect of SBOS on growth performance of Cherry Volley Duck(1) BW of ducks in experiment groups were not higher than control group in 1-14d period, but BW of ducks in group 4 was increased and the differences were not significant (P>0.05) in 15-28d period. (2) ADG of ducks in group 4 only was greater than control group in the whole experiment period, but the differences were not significant (P>0.05). (3) ADFI of ducks in group3,4 and 5 were lower than control group in the whole experiment period, and group 5 was lower significantly than control group (P<0.05). (4) F/G of ducks in group 3,4 and 5 were higher than control group in 15-28d period, and group 4 and 5 were greater significantly (P< 0.05) than control group. (5) Significant differences in BW, ADG and ADFI between group 2 and control group were not be observed, but F/G of ducks in group 2 was greater significantly (P<0.05) than control group.2. Effect of SBOS on Escherichia coli in intestinal tract of Cherry Volley DuckThe inhibit effect of SBOS on Escherichia coli in intestinal tract of Cherry Volley Duck was obvious, but the inhibit effect was not be observed in the first week; The inhibit effect in group 3,4 and 5 were higher than control group, and group 3 obtained significant level(P<0.05)at the second weekend; The inhibit effect in group 3,4 and 5 were all greater significantly (P<0.05) than control group at the third weekend; The inhibit effect in group 3,4 and 5 were all greater than control group at the fourth weekend, group 3 and 5 were greater significantly (P<0.05) than control group; The inhibit effect in group 2 was observed until the second weekend and obtained significant level (P<0.05) in 15-28d period.3. Effect of SBOS on Bifidobacterium in intestinal tract of Cherry Volley DuckThe proliferation effect of SBOS on Bifidobacterium was observed in the whole experiment period. The proliferation effect in group 3,4 and 5 were higher than control group in 1-14d period, but the differences were not significant (P>0.05); The proliferation effect in group 3,4 and 5 were all greater than control group in 15-28d period, and group 4 was obtained significant level (P<0.05). The proliferation effect in group 2 was greater than control group in the whole experiment period, but significant differences were not be found (P>0.05)4. Effect of SBOS on Lactobacillus in intestinal tract of Cherry Volley DuckThe proliferation effect of SBOS on Bifidobacterium was observed in the whole experiment period. The proliferation effect in group 3 and 4 were greater significant (P<0.05) than control group in 15-28d period, and group 5 and 2 were higher than control group, but significant differences were not be observed (P>0.05) in the whole experiment period.In summary, SBOS added in proper dosage can improve F/G, and regulate the microbial flora effectively, inhibit the growth of harmful bacterium and proliferate the good bacteria in intestinal tract of Cherry Volley Duck.