| The research discusses the Possibiliy of chlorogenic acid as immunoenhance. This research confirmed the using method and doesage of chlorogenic acid primarily and provided certain theories foundation for the using in the chinese soft-shelled turtle.This research has two parts totally, the first part studied the influence of chlorogenic acid to the item growth and several non-specific immunity factors, to ascertain the dosage.The chinese soft-shelled turtle were fed dies containing o(control),100mg/kg,200mg/kg, 400mg/kg,800mg/kg, chlorogenic acid to ensurate the number of leucocytes, the content of lysozyme, content of complement C3,C4 in plasma; phagocytic percentages, phagocytic index of leukocyte and erythrocytes in blood.The relative growth rate of 200 mg/kg dose group was significantly higher (P<0.01), while 0 mg/kg dose group was significantly lower (P<0.05), and C(200mg/kg),D (400mg/kg),E(800mg/kg)mg/kg dose group was significantly higher than the control group (P<0.05).the length of duodenum:C (200 mg/kg,D (400mg/kg),E(800mg/kg)were significantly higher than control group(P<0.01).The heavith of liver were no significantly efficient. Chlorogenic acid was activity efficient to non-special immunity of the Chinese soft-shelled turtle,for example:content of lysozyme (100mg/kg),C (200mg/kg),D (400mg/kg),E (800mg/kg) were significantly higher than control group(P<0.01)p.Meanwhile content of complement C3 (200mg/kg) significantly higher than E group (800mg/kg) (P<0.05);and content of complement C4 (200mg/kg) and D4 (400 mg/kg) significantly higher than A group (P<0.01).All in all, A chlorogenic acid promotes the growth gastrointestinal tract,Decreas food conversion ratio, increase the young Chinese soft-shelled turtle. B It can also improve non-specific immunity of young Chinese soft-shelled,for example,the kidney, Phagocytic percentages of leukocyte, lysozyme,the content of completement C3. C The best suitable content of chlorogenic acid is 200-400mg/kg.
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