Young Tea Plants Gene Expression Analysis Under Drought Stress

Tea plant (Camellia sinensis (L.) O. Kuntze) is one of the important economic plants in China, which had long planting history and distributes widely. It has been proved that Southwest of China is the origin of tea plant, having rich of tea resources and good foundation for breeding. Drought is the main environmental limit factors in the world's crop production, one of the world's arid and semi-arid regions account for about 36% of the total land area, accounting for 43% of arable land. China's arid and semi-arid land area accounting for about 1/2, while, with the enhancement of Greenhouse effect, the of drought-resistant varieties breeding will be an important aspect in the future. The traditiong way of tea breeding is maine system breeding and plant breeding,but there are some drawback like genetic backgroud complex,breeding instability,needing long time and other defects,while the drought indicators are mostly based on environmental characteristics. Molecular breeding can effectively avoid these problems for it directly screening genetic material DNA. DD-PCR (differential display PCR, or DDRT-PCR) have advantages of simple,high sensitivity and efficiency.It is first time that study gene differentially expressed under arid environment.This experiment try to screen different exprement gene to drought-resistant index,intended to make some contribution to drought variety, and got results as followed:1. The best part extracting RNA from young tea using kit is leaves and new roots,because its have less secondary products to pollution of the extracted RNA.But RNA from roots difficult to preservation as the same environment,rapid degradation,illustrating the exracting RNA with kit had other impurities affect the preservation of root RNA.2.Amplification reaction with Mixmixture better than conventional Taq enzyme system in PCR; gel electrophoresis is 120mv;electrophoresis time about 1h;separation gel concentra-tion about1.5%;experimentparts part is leaves;the annealing temperature 45℃,when meeting these codition getting better result. When experiments parts is roots,we need put down.Annealing temperature.PCR reaction parameters is like ordinary PCR reaction.3.There are three changes afer PCR:up expression,down expression and constant expression. Some bands have mergers on anchor primers.Identified anchor primers and random premers combinations after screening.Need to determine the stress time when experiment part is young roots.4.There are many gene bands expression changed after drought stress from sequencing results,and some bands changed trend similar other researchers reported.5.Making sure this method and technique is feasible in studying anti-drought molecular nechanism on the tea,for example,we achieved good experiments results in FuDing seeding,so the method can be applied to research other tea varieties and different breeding statement of tea.