Effect Of Qinkangsan On Cellular Infectivity Of NDV Proliferation And NO Secretion Of SPL

New Castle disease is an acute and highly contagious disease caused by Newcastle disease virus, which do great harms to the poultry industry and has not been controlled effectually till today. Earlier period studies indicated that Complex Chinese crude drug Qinkangsan had good therapeutic effect. Based on these studies, the purpose of this topic is to survey the cytobiology mechanism of Qinkangsan for clinical application and advanced development.The experiment and consequence as follows:1. Effect of Qinkangsan on CEF proliferation Qinkangsan(1g/ml) was diluted into 2-2,2-3,2-4,2-5,2-6,2-7,2-8,2-9,2-10g/ml concentrations and used for CEF (0.1ml each hole).The effects of drug was judged according to OD value.The reasults showed that Qinkangsan could promote cell proliferation obviously in concentrations of 2-6,2-7,2-8 g/ml and restrain cell proliferation obviously in concentrations of 2-2,2-3,2-4g/ml.The maximal safety concentration is 2-5g/ml.2. Effect of Qinkangsan on cellular infectivity of virus Three concentrations(2-5,2-7,2-9g/ml) of Qinkangsan(0.1ml) and NDV(200 TCID50) were respectively added into CEF in three different ways [synchronously(sterilization group), before(prophylaxis group) and after(cure group) inoculation of virus].Cell proliferation were tested by The effects of Qinkangsan on infection of virus to CEF were evaluated by OD value's changes.The result shows that virus inhibition in sterilization group, prophylaxis group and cure group was 0.794,0.525 and 0.474 respectively.3. Effect of Qinkangsan on SPL proliferation Qinkangsan(lg/ml) was diluted into 2-2,2-3,2-4,2-5,2-6,2-7,2-8,2-9,2-10g/ml concentrations and used for SPL(0.1ml each hole).The effects of drug was judged according to OD value.The reasults showed that Qinkangsan could promote cell proliferation obviously in concentrations of 2-4,2-5,2-6,2-7g/ml and restrain cell proliferation obviously in concentrations of 2-2g/ml. 4. Effect of Qinkangsan on NO secretion of SPL Qinkangsan was diluted into 2-2,2-5,2-8 g/ml concentrations and used for SPL(0.1ml each hole). Contents of NO and NOS in culture solution were determined by kits to judge effects of Qinkangsan on NO secretion. The reasults showed that Qinkangsan of all concentrations could increase contents of NO and NOS, result of 2"2g/ml group(0.1ml 25mg) was most obviously.