| There are many reports that before the Newcastle disease virus in SPF chicken embryos results in the proliferation of the virus is much higher than those in ordinary low-free chicken embryo, for avian influenza virus in SPF chicken-free chicken and low contrast between the still relatively rare. And this experiment with recombinant avian influenza virus (H5N1 subtype Re-5 strain) as the research object to simulate avian influenza inactivated vaccine production, through SPF chicken embryos were used and the general production of raw materials for the production of inactivated oil emulsion preparation of seedlings , from production to the final product at all stages of the immune effect of a full course of the data were analyzed. SPF chicken embryos with the normal production of maternal antibodies and allantoic fluid sterile test comparison we can see normal production chicken varying degrees of maternal antibodies do not exist in SPF chicken embryos, normal embryo production results of three sampling only 1 / 3 sterile growth, while the SPF chicken sampling results of the three batches were 3 / 3 sterile growth. Through different kinds of drug dilutions inoculated SPF chicken embryo test we can see that SPF chicken embryos inoculated with the kinds of poisoning by selected dilution of 1 / 1 million as the best inactivated vaccine production can be achieved with the standard strains, and can improve the use of egg rate, lower production costs. Consistent with a low pathogenicity avian influenza virus features, pairs of birds and mammals, a high degree of safety, immunization of high effectiveness, and suitable for the use of a large number of the proliferation of chick embryo. This test uses hand-inoculation, harvested chorioallantoic fluid, filtered through the first impurity addition to macromolecules in order to avoid large particles allantoic fluid molecules inactivated agent consumption. In order to minimize the formaldehyde inactivated agent to add volume but also ensures that the inactivation of antigen test and sterile test pass tests of conventional antigen and sample antigen, respectively, 0.15% and 0.20% select two concentrations of formaldehyde inactivated, in SPF chicken embryos can be produced by testing antigen production than the ordinary chicken embryo production of conventional antigen to achieve a lower formaldehyde content inactivated purposes. Semi-pilot test data comparison before antigen inactivate HA, inactivated HA and EID50 3 data are superior to conventional antigen. Ordinary production and SPF chicken embryos for the production of raw materials simulated operation of avian influenza inactivated vaccine antigen and make a variety of craft production emulsifier, in accordance with the design formula, mixing with the emulsion pump sufficient oil emulsion made from inactivated vaccine, After physical properties testing, the system of two kinds of vaccines are milky white water in oil formulations, the viscosity is small, uniform stability. Through the sterile and safety inspection, no chickens any adverse effects to prove that the two vaccines are safe for testing the chicken. SPF chicken embryos to produce the pilot production of inactivated vaccine with an ordinary chick embryo production of conventional inactivated vaccine compared to the various parts of HI antibody were higher than the latter. The duration of the immune antibody Variation of the analysis found that the test vaccine is more stable HI antibody immune response in line with the requirements of oil-emulsion inactivated vaccine. Through the inspection of raw materials, semi-finished and finished product testing in three stages of testing data comparison for the present, China's Production of avian influenza inactivated vaccine antigen titer increased provision of technical reference for the future development of avian influenza inactivated vaccine, to seek new ways to improve the quality to provide test data, promoting China's veterinary biological products research and development.
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